BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0548100 Os10g0548100|AK107224
         (277 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G52420.1  | chr5:21281817-21282545 FORWARD LENGTH=243          104   4e-23
AT1G27990.1  | chr1:9752799-9753919 REVERSE LENGTH=272             73   2e-13
AT5G23920.1  | chr5:8073363-8074118 REVERSE LENGTH=230             63   2e-10

>AT5G52420.1 | chr5:21281817-21282545 FORWARD LENGTH=243
          Length = 242

 Score =  104 bits (260), Expect = 4e-23,   Method: Compositional matrix adjust.
 Identities = 71/213 (33%), Positives = 92/213 (43%), Gaps = 12/213 (5%)

Query: 43  FSIKQLNSFGAAAVLAFSTTVPLSDIAFALLVIPYLVVLSVLAFPQRPGKPNPGAPVFLG 102
           FS +QLN      VL+ S  V + D  F +L + Y   LS L FP     PN  AP+   
Sbjct: 40  FSFRQLNILAIIIVLSASGLVTIQDFIFTILTLIYFFFLSKLIFPPH-NNPNRDAPLTSS 98

Query: 103 RGRFLLRAHDALGFLVGAALPALYILDGLRSGDTAGVXXXXXXXXXXXXQIFTEGLAAAW 162
             + + R +     +VG  +P  YI +G+   D  GV            QIF EGLA  +
Sbjct: 99  TNK-IFRIYVTAAGIVGLIIPICYIFEGIVEDDKNGVSAAAPHVFLLASQIFMEGLATMF 157

Query: 163 PGRFSLPVRAAVVVMYGARRMFAASEWLRQEMEKRDQFXXXXXXXXXXXXXXXXXXXXXX 222
              FS P R  V ++Y ARR+    EW+  E  + D                        
Sbjct: 158 --GFSAPARILVPIVYNARRVLTLVEWIMSEFSRED-------VTGTVSARRMYAGKVLA 208

Query: 223 XXNLAYWGINLFAFLLPFYLPKAFNRYYCGSDS 255
             NL  W  NLF  L+P YLP+AF RYY GSD 
Sbjct: 209 AANLGIWSFNLFGVLIPVYLPRAFKRYY-GSDK 240



>AT1G27990.1 | chr1:9752799-9753919 REVERSE LENGTH=272
          Length = 271

 Score = 72.8 bits (177), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 62/205 (30%), Positives = 85/205 (41%), Gaps = 10/205 (4%)

Query: 53  AAAVLAFSTT--VPLSDIAFALLVIPYLVVLSVLAFPQRPGKPNPGAPVFLGRGRFLLRA 110
            A +L FS +  VP+ D+ F      Y++ LS LAFP   G       VF  RG  L R 
Sbjct: 51  VAFILVFSASGLVPILDMLFPAFASIYIIALSRLAFPSH-GVSTASPEVF--RGSKLFRL 107

Query: 111 HDALGFLVGAALPALYILDGLRSGDTAGVXXXXXXXXXXXXQIFTEGLAAAWPGRFSLPV 170
           +   G  +G  LP  Y+L G   GD   V            QI TE + +     FS PV
Sbjct: 108 YVISGTTIGLFLPLAYVLGGFARGDDHAVRSATPHLFLLSCQILTENVISGL-SLFSPPV 166

Query: 171 RAAVVVMYGARRMFAASEWLRQEMEKRDQFXXXXXXXXXXXXXXXXXXXXXXXXNLAYWG 230
           RA V ++Y   R+F    W +     +                           NL Y+G
Sbjct: 167 RALVPLLYTVWRIFVIIGWSKDVWFNKS----LPINATPNVVTWFWFGRYLALANLGYFG 222

Query: 231 INLFAFLLPFYLPKAFNRYYCGSDS 255
           +NL  FL+P +LP+AF +Y+   D 
Sbjct: 223 VNLLCFLIPRFLPRAFEQYFRERDE 247



>AT5G23920.1 | chr5:8073363-8074118 REVERSE LENGTH=230
          Length = 229

 Score = 62.8 bits (151), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 59/216 (27%), Positives = 86/216 (39%), Gaps = 25/216 (11%)

Query: 46  KQLNSFGAAAVLAFSTTVPLSDIAFALLVIPYLV-VLSVLAFP-----QRPGKPNPGAPV 99
           +QL       +LA    V + +IAF +L   YL   LS  AFP     Q+    NP   +
Sbjct: 31  RQLVFLSFMILLAAKGLVGIGEIAFVILCYIYLYEFLSRFAFPRKQTEQKKRLSNPKNKL 90

Query: 100 FLGRGRFLLRAHDALGFLVGAALPALYILDGLRSGDTAGVXXXXXXXXXXXXQIFTEGLA 159
           F  +  FL  A      ++G   P  YI DG+  GD  G             Q FTE + 
Sbjct: 91  F--QAYFLATA------IIGLLFPLCYIGDGIYRGDIHGAGAAAPHLFLLSGQAFTEPIG 142

Query: 160 AAWPGRFSLPVRAAVVVMYGARRMFAASEWLRQEMEKRDQFXXXXXXXXXXXXXXXXXXX 219
             +  ++S+P+     V Y ARR+FA  +W++ E     +                    
Sbjct: 143 --FSDKYSMPIGILGPVFYNARRIFALLDWVKAEFSDTQR---------PGGPLRLYGGR 191

Query: 220 XXXXXNLAYWGINLFAFLLPFYLPKAFNRYYCGSDS 255
                N   W  NLF  LLP +LP++   Y+ G + 
Sbjct: 192 VIASVNTVMWFYNLFGLLLPVFLPRSCEIYFSGDNK 227



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.328    0.142    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,948,078
Number of extensions: 121731
Number of successful extensions: 303
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 298
Number of HSP's successfully gapped: 3
Length of query: 277
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 180
Effective length of database: 8,447,217
Effective search space: 1520499060
Effective search space used: 1520499060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 111 (47.4 bits)