BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0539400 Os10g0539400|AK071222
         (351 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G03210.1  | chr3:740987-742093 FORWARD LENGTH=369              234   5e-62

>AT3G03210.1 | chr3:740987-742093 FORWARD LENGTH=369
          Length = 368

 Score =  234 bits (597), Expect = 5e-62,   Method: Compositional matrix adjust.
 Identities = 139/357 (38%), Positives = 188/357 (52%), Gaps = 26/357 (7%)

Query: 17  LFVPMGLAGWHLSRNKVLFFSGALFVSLAVGVXXXXXX---XXXXXXXXXXXXXXXXIXX 73
           LFVPM +AGWHLSRNK+LFFSGALF+SLAV V                         I  
Sbjct: 16  LFVPMAMAGWHLSRNKMLFFSGALFISLAVCVHLTPYFPSVSDIVASVSSVVVYDHRISC 75

Query: 74  XXXXXXXXXXCVPLLHRVSWADAGGESGVG---RAWAWPPSLAS-TCGLARLSRDDASLL 129
                       P+ +  S     G + +    + W W  S    +C   +L + D S L
Sbjct: 76  INEVNQIVWDVKPVPNPESVRRNNGSTKLDYFVKNWDWMKSRKVLSCEFQKLDKFDVSDL 135

Query: 130 LNGSWVMVAGDSQXXXXXXXXXXXXXXXXXXXXXXXX--XFRRHSDYRATVPARGISVDF 187
           LNGSWV+VAGDSQ                           FRRHSDY   V   G+ +DF
Sbjct: 136 LNGSWVVVAGDSQARFVALSLLNLVLGSDSKAMDSVRGDLFRRHSDYSIVVKEIGMKLDF 195

Query: 188 VWAPFESNLTRLLHEDLRLAPRTPDVLVLGSGLWHMLHVTDAARYGDALASVVDAAKSLR 247
           VWAP+E +L  L+    +   + PDV+++G+GLWHMLHV +A+ +G  L  +    +SL 
Sbjct: 196 VWAPYEKDLDDLV-VSYKKMKKYPDVVIMGTGLWHMLHVNNASDFGFRLRQLSSHVESL- 253

Query: 248 SPLPVPPP-------------HMFWLGLPLLVNHMLNTDAKKVHMNDTILQAYDLEVEQR 294
             +P+ P              H+FW+G+P+L+N MLNTD KK  M+DT+   YD  + + 
Sbjct: 254 --VPLTPKEQEGGGSVSGRSVHLFWIGMPVLINGMLNTDEKKEKMSDTVWHEYDRSLGES 311

Query: 295 GLLQRDGGPFLLLDVGKLSRGCGQQCTADGMHYDGDVYDAVLHIMLNALVIESQQRI 351
            +L++ GGP +LLD+   +  CG QCT DGMHYD  VYDA +H+MLNAL+IES Q +
Sbjct: 312 KILRQMGGPLILLDIQSFTWNCGPQCTLDGMHYDSAVYDAAVHVMLNALLIESHQSL 368



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.139    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,184,480
Number of extensions: 224348
Number of successful extensions: 697
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 694
Number of HSP's successfully gapped: 2
Length of query: 351
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 251
Effective length of database: 8,364,969
Effective search space: 2099607219
Effective search space used: 2099607219
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 112 (47.8 bits)