BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0493800 Os10g0493800|AK108599
         (283 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G53270.1  | chr3:19750406-19752168 REVERSE LENGTH=281          270   5e-73

>AT3G53270.1 | chr3:19750406-19752168 REVERSE LENGTH=281
          Length = 280

 Score =  270 bits (691), Expect = 5e-73,   Method: Compositional matrix adjust.
 Identities = 128/286 (44%), Positives = 198/286 (69%), Gaps = 14/286 (4%)

Query: 1   MDLSPFKLDIDELLADYTEANCTAFADFKRLWMAKKFSYIYEGRPKTNSGAFMQSLFLHC 60
           M+LSPFK DIDEL+ ++ E + T FAD K +W+++KFS+IYE  P +N   FMQSL++H 
Sbjct: 1   MNLSPFKRDIDELIDEFVEDDLTTFADMKSVWLSRKFSHIYEASPNSNLAFFMQSLYVHT 60

Query: 61  IGHMTSQSCMPQRLAGLYCLYCLYESQPYKPHFKIYLSLEELKKLKDFVVEAKQNGMAVV 120
           IGHM S     +RL GLYCLYCL+E QP+KP F+IY+SL+EL K +D VVEAK  G+ + 
Sbjct: 61  IGHMVSIDSFSRRLGGLYCLYCLHEIQPFKPKFRIYISLQELGKFRDLVVEAKDKGVEIA 120

Query: 121 PSLVKRMLDKGMILFGFINLLGDSGVKQVNELTASQNKRVKFACDKLFMNTQIDSYMHMD 180
            ++ K+MLD+ M++FG ++    S  K +++LT  QN RV+FA +KL  +T I+ ++H+D
Sbjct: 121 AAVAKKMLDENMLIFGAVD--ETSATKTIHQLTELQNARVRFAYEKLICDTSINQFIHLD 178

Query: 181 LGSELELDKIKKSSLDYAKAKELAFAEASNIIDVEDARHIVQNDKLLGDRVDEIVKEWDA 240
           +G E++L+ + K S++YA+AK+ A   A  I++++D +HI +  +L+G+R++++ +EWD+
Sbjct: 179 MGKEVDLNSLDKMSIEYAEAKKRAVEGAGEIMEIDDIKHISEEKELMGERMEKLKEEWDS 238

Query: 241 QKEAFYEKT-----GVRRDELAVVEHDESGVLPHENDEFDEIRQLL 281
           Q+  FYE+T          +L  VEHD       E+D FDE+ +LL
Sbjct: 239 QRLYFYEQTKLDGFTTTPKQLTNVEHD-------EDDGFDELDRLL 277



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.136    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,066,350
Number of extensions: 254515
Number of successful extensions: 712
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 711
Number of HSP's successfully gapped: 1
Length of query: 283
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 186
Effective length of database: 8,447,217
Effective search space: 1571182362
Effective search space used: 1571182362
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)