BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0485300 Os10g0485300|J013001D01
         (377 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G33630.1  | chr4:16155560-16159094 FORWARD LENGTH=685          128   5e-30
AT1G27510.1  | chr1:9554945-9558293 REVERSE LENGTH=652             79   4e-15

>AT4G33630.1 | chr4:16155560-16159094 FORWARD LENGTH=685
          Length = 684

 Score =  128 bits (321), Expect = 5e-30,   Method: Compositional matrix adjust.
 Identities = 110/392 (28%), Positives = 177/392 (45%), Gaps = 43/392 (10%)

Query: 2   LYIAFQLQLRTAVYREDFRSAHKLKLAIAATSKNDTVGRAISDLN--------------- 46
           L    + QL  A+ RED+  A +LK+AIAAT+ ND VG+ +S                  
Sbjct: 129 LLSVLKSQLNRAIKREDYEDAARLKVAIAATATNDAVGKVMSTFYRALLEERYKDAVYLR 188

Query: 47  -------LGWWSGISGNLSDPYGLIIRISAEHGRYVAKSYDTRQLNSDGPGFPIFEIYFA 99
                  +GWWSGIS ++ DP+GLI++I+AEHGRYVA+SY+ RQL++   G P+FEI+  
Sbjct: 189 DKAGAGLVGWWSGISEDVKDPFGLIVQITAEHGRYVARSYNPRQLSTSAAGAPLFEIFLT 248

Query: 100 -EANGGYNLQAVHLKPDDSDSQQLSNTLREKLGMDSINISSSSF----GAKHEDHNEGVN 154
            +  G Y  QAV+LK              +   M S  ++   F    G K +  N  V 
Sbjct: 249 LDGKGNYKKQAVYLK--------WKEIFPDVPTMPSRTLTPGRFLTSPGRKEDTGNLAVE 300

Query: 155 MDDQNSDDSDISAG-----PAGFKNLPSDSTPVPRVKILKVVPMENVNQDYIIKIFDQMS 209
             +    D+           +GF++   D  P  +VK++KV     V++D+I K+ +Q++
Sbjct: 301 SSEDEESDNSDDDSDLLEESSGFQSFLRDMIPGVKVKVMKVTAPGRVDKDFISKVIEQIA 360

Query: 210 XXXXXXXXXXXXXXXXXXXXXXXNVEEAEAASAEDNVDESGDESDIEALISIDFITEDDK 269
                                    E+      E   DE  D +     I++ F+  D  
Sbjct: 361 DEEDEENDLDIEDIDVEDDTKAEIDEKNADIELESVTDEIIDNNGGRE-IAVKFVIGDIV 419

Query: 270 DFMSPSST-KAFERMPARLERRDRFSFSFYTEQYSKRQDVEKVQGIS-KEKVGLRTAQQD 327
           D +S +   K   R PA LE  +  SF    E+    ++ + V+G +  +  G R +++ 
Sbjct: 420 DRLSGNQPLKESLRSPANLESVENSSFYLRLEKDLNVKESKGVEGTTLVDGKGSRQSRRR 479

Query: 328 DDDLQFDRVKLVGSNRKLSVVFRAKVGKHYQL 359
            +++  D  K +   +K+SV     VG+   L
Sbjct: 480 IENIMGDLAKSIEKEKKISVKMLKDVGELLSL 511



>AT1G27510.1 | chr1:9554945-9558293 REVERSE LENGTH=652
          Length = 651

 Score = 79.0 bits (193), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 42/128 (32%), Positives = 66/128 (51%), Gaps = 23/128 (17%)

Query: 9   QLRTAVYREDFRSAHKLKLAIAATSKNDTVGRAISDLN---------------------- 46
           QL  AV +EDF  A KLK AI+  + +D V   +  L                       
Sbjct: 112 QLEDAVEKEDFEEAVKLKQAISEATVDDAVAEIMRQLQTAVNEERYHDASRLCNETGSGL 171

Query: 47  LGWWSGISGNLSDPYGLIIRISAEHGRYVAKSYDTRQLNSDGPGFPIFEIY-FAEANGGY 105
           +GWW G+  +  +P+G I+ I+   GR++ KSY  RQL ++  G P+FEI+   + +GGY
Sbjct: 172 VGWWVGLPRDSEEPFGRIVHITPGVGRFIGKSYSPRQLVAEAAGTPLFEIFVIKDTDGGY 231

Query: 106 NLQAVHLK 113
            +Q V+++
Sbjct: 232 VMQVVYVQ 239



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,846,763
Number of extensions: 335951
Number of successful extensions: 902
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 898
Number of HSP's successfully gapped: 2
Length of query: 377
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 277
Effective length of database: 8,364,969
Effective search space: 2317096413
Effective search space used: 2317096413
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 112 (47.8 bits)