BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0540500 Os09g0540500|AK120729
         (414 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G02850.1  | chr5:652159-653439 FORWARD LENGTH=427              143   2e-34

>AT5G02850.1 | chr5:652159-653439 FORWARD LENGTH=427
          Length = 426

 Score =  143 bits (360), Expect = 2e-34,   Method: Compositional matrix adjust.
 Identities = 97/250 (38%), Positives = 125/250 (50%), Gaps = 16/250 (6%)

Query: 84  NLFELSPSRAAWISAYRGXX-XXXXXXXXXXXXXXXXXXXXXXKEALSLLNTLQTQLFEA 142
            LF++SP+RA W+SA+RG                         KE LS  N+LQTQLFEA
Sbjct: 99  KLFDVSPNRAIWLSAFRGSLPSFLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEA 158

Query: 143 VAELQETXXXXXXXXXXXXXXXXXXXSILAFAKKLREAHHVLDRLVDDYADYRRDPKRPR 202
           V ELQE                    S+LAFA KL++A  VLD LVDDY+DYR+ PKR +
Sbjct: 159 VTELQEILDLQDAKQKVAREIKSKDSSLLAFANKLKDAERVLDMLVDDYSDYRK-PKRSK 217

Query: 203 GAAAADDPEPVSDGDFGASLHSKLNLDDVLTYAHRISYTTFAPPEHXXXXX-XXXXXXXX 261
                D+           ++ S+L L D+L YAH+ISYTTFAPPE               
Sbjct: 218 -IEEDDEDNDNESSSSSTTVSSQLKLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPA 276

Query: 262 XQENEMRMSQLYQFADLDVGVPKSQEAKERTAAEGDATPLFQPSPTQEAA-------VLP 314
            Q+ +MR SQLY FADLD+G+PK+ E  E+         L +P P  EA        +LP
Sbjct: 277 PQDEQMRASQLYTFADLDIGLPKTVENMEKKVEA-----LIEPPPPPEAMDISAIHNLLP 331

Query: 315 ITVPHPHGWR 324
             +  P GW+
Sbjct: 332 PNIAVPSGWK 341



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.131    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,269,461
Number of extensions: 163015
Number of successful extensions: 249
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 245
Number of HSP's successfully gapped: 1
Length of query: 414
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 313
Effective length of database: 8,337,553
Effective search space: 2609654089
Effective search space used: 2609654089
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 113 (48.1 bits)