BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0528300 Os09g0528300|AK121125
         (190 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G26665.2  | chr1:9214510-9215426 FORWARD LENGTH=190            242   1e-64
AT5G41910.1  | chr5:16778160-16779032 REVERSE LENGTH=187          224   2e-59

>AT1G26665.2 | chr1:9214510-9215426 FORWARD LENGTH=190
          Length = 189

 Score =  242 bits (617), Expect = 1e-64,   Method: Compositional matrix adjust.
 Identities = 115/156 (73%), Positives = 138/156 (88%)

Query: 34  DASKQNLALMMASIQRTLGLLHQLNLNVSSFSSASQLPLLQRLNSLVAELDTMQKHAEGC 93
           D SK+NL+ ++ SI++TLG+LHQL+L V+SF+ ASQL LLQRLNSLV ELD M K +E C
Sbjct: 33  DDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKC 92

Query: 94  NIQVPMEVVNLIDDGKNPDEFTRDVLNSCIAKNQVTKGKTDAFKSLRKHLLEELEQAFPE 153
           NIQ+PMEV+NLIDDGKNPDEFT+DVLNSCIA+NQVTKGKTDAFK LRKH+LEELEQ FP+
Sbjct: 93  NIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPD 152

Query: 154 DVEAYREIRATAAAESKQLAQSQSALPNGDVKVKPE 189
           +V+ YREIRA++AA +K+LAQSQS LPNGD KVK E
Sbjct: 153 EVDMYREIRASSAAVTKRLAQSQSVLPNGDAKVKNE 188



>AT5G41910.1 | chr5:16778160-16779032 REVERSE LENGTH=187
          Length = 186

 Score =  224 bits (571), Expect = 2e-59,   Method: Compositional matrix adjust.
 Identities = 112/157 (71%), Positives = 132/157 (84%), Gaps = 1/157 (0%)

Query: 34  DASKQNLALMMASIQRTLGLLHQLNLNVSSFSSASQLPLLQRLNSLVAELDTMQKHAEGC 93
           D S + L  ++ S Q+ L LLHQL L VSSF+ ASQL LLQRLNSLV ELD M K ++ C
Sbjct: 29  DDSNEKLNQIINSNQKILELLHQLKLTVSSFTPASQLHLLQRLNSLVMELDNMAKLSDKC 88

Query: 94  NIQVPMEVVNLIDDGKNPDEFTRDVLN-SCIAKNQVTKGKTDAFKSLRKHLLEELEQAFP 152
           NIQVP+EV+NLIDDGKNPDEFT+DVLN +CIAKNQVTKGK+DAFK LRKHLLEELEQAFP
Sbjct: 89  NIQVPIEVLNLIDDGKNPDEFTKDVLNKNCIAKNQVTKGKSDAFKGLRKHLLEELEQAFP 148

Query: 153 EDVEAYREIRATAAAESKQLAQSQSALPNGDVKVKPE 189
           ++V+ YR+IRA+ AAE+K+LAQ+QS LPNGD KVK E
Sbjct: 149 DEVDRYRDIRASYAAEAKRLAQTQSVLPNGDAKVKSE 185



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.126    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,032,049
Number of extensions: 111893
Number of successful extensions: 351
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 350
Number of HSP's successfully gapped: 2
Length of query: 190
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 97
Effective length of database: 8,556,881
Effective search space: 830017457
Effective search space used: 830017457
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 109 (46.6 bits)