BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os09g0507800 Os09g0507800|AK061725
(258 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G62870.1 | chr3:23242862-23244273 REVERSE LENGTH=257 369 e-103
AT2G47610.1 | chr2:19529854-19531401 FORWARD LENGTH=258 357 4e-99
>AT3G62870.1 | chr3:23242862-23244273 REVERSE LENGTH=257
Length = 256
Score = 369 bits (947), Expect = e-103, Method: Compositional matrix adjust.
Identities = 181/258 (70%), Positives = 200/258 (77%), Gaps = 2/258 (0%)
Query: 1 MAPKRGGRAPVPAKKKTEKVTNPLFEKRPKQFGIGGALPPKKDLHRFVKWPXXXXXXXXX 60
MAPK+G + V +KKK EKVTNPLFE+RPKQFGIGGALPPKKDL R++KWP
Sbjct: 1 MAPKKGVK--VASKKKPEKVTNPLFERRPKQFGIGGALPPKKDLSRYIKWPKSIRLQRQK 58
Query: 61 XXXXXXXXXPPALNQFTRTLDKNLATNLFKMLLKYRPEDXXXXXXXXXXXXXXEAEGKTV 120
PPALNQFT+TLDKNLAT+LFK+LLKYRPED EAEGK
Sbjct: 59 RILKQRLKVPPALNQFTKTLDKNLATSLFKILLKYRPEDKAAKKERLLNKAQAEAEGKPA 118
Query: 121 EAKKPIVVKYGLNHVTYLIEQSKAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGKA 180
E+KKPIVVKYGLNHVTYLIEQ+KAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGK+
Sbjct: 119 ESKKPIVVKYGLNHVTYLIEQNKAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGKS 178
Query: 181 RLGSIVHKKTASVLCLTTVKNEDKLEFSKILEAIKANFNDKFDEVRKKWGGGVMGSKSQX 240
RLG++VH+KTA+ LCLTTVKNEDKLEFSKILEAIKANFNDK++E RKKWGGG+MGSKSQ
Sbjct: 179 RLGAVVHQKTAAALCLTTVKNEDKLEFSKILEAIKANFNDKYEEYRKKWGGGIMGSKSQA 238
Query: 241 XXXXXXXXXXXXXXQRMT 258
QRM
Sbjct: 239 KTKAKERVIAKEAAQRMN 256
>AT2G47610.1 | chr2:19529854-19531401 FORWARD LENGTH=258
Length = 257
Score = 357 bits (916), Expect = 4e-99, Method: Compositional matrix adjust.
Identities = 172/242 (71%), Positives = 188/242 (77%)
Query: 17 TEKVTNPLFEKRPKQFGIGGALPPKKDLHRFVKWPXXXXXXXXXXXXXXXXXXPPALNQF 76
EKV+NPLFE+RPKQFGIGGALPPKKDL R++KWP PPALNQF
Sbjct: 16 AEKVSNPLFERRPKQFGIGGALPPKKDLSRYIKWPKSIRLQRQKRILKQRLKVPPALNQF 75
Query: 77 TRTLDKNLATNLFKMLLKYRPEDXXXXXXXXXXXXXXEAEGKTVEAKKPIVVKYGLNHVT 136
T+TLDKNLAT+LFK+LLKYRPED EAEGK E+KKPIVVKYGLNHVT
Sbjct: 76 TKTLDKNLATSLFKVLLKYRPEDKAAKKERLVKKAQAEAEGKPSESKKPIVVKYGLNHVT 135
Query: 137 YLIEQSKAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGKARLGSIVHKKTASVLCL 196
YLIEQ+KAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGK+RLG++VH+KTAS LCL
Sbjct: 136 YLIEQNKAQLVVIAHDVDPIELVVWLPALCRKMEVPYCIVKGKSRLGAVVHQKTASCLCL 195
Query: 197 TTVKNEDKLEFSKILEAIKANFNDKFDEVRKKWGGGVMGSKSQXXXXXXXXXXXXXXXQR 256
TTVKNEDKLEFSKILEAIKANFNDK++E RKKWGGG+MGSKSQ QR
Sbjct: 196 TTVKNEDKLEFSKILEAIKANFNDKYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQR 255
Query: 257 MT 258
M
Sbjct: 256 MN 257
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.318 0.135 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,611,960
Number of extensions: 165825
Number of successful extensions: 384
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 382
Number of HSP's successfully gapped: 2
Length of query: 258
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 162
Effective length of database: 8,474,633
Effective search space: 1372890546
Effective search space used: 1372890546
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)