BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0476000 Os09g0476000|AK073131
         (265 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G08120.1  | chr5:2600743-2602678 REVERSE LENGTH=327             89   2e-18

>AT5G08120.1 | chr5:2600743-2602678 REVERSE LENGTH=327
          Length = 326

 Score = 89.4 bits (220), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 63/197 (31%), Positives = 108/197 (54%), Gaps = 13/197 (6%)

Query: 75  QREELAALQEQLSGLQKKLLEKDEALRSAEHLISRISAANAAVDELRGQLTEKESQIEST 134
           ++EE++ L+EQ++ LQ KL EK+E L+S E   ++++     ++     + EK+  I+S 
Sbjct: 135 EKEEMSRLREQVNDLQTKLSEKEEVLKSMEMSKNQVNEIQEKLEATNRLVAEKDMLIKSM 194

Query: 135 GSELHGAKIQLAEKQAALEKLEWEAKVSSTKVEELQVDVASMDVEISALMKLFRKITEND 194
             +L   KI+LA+KQAALEK +WEAK + T+  +LQ  + +++ +IS   ++F  + + D
Sbjct: 195 QLQLSDTKIKLADKQAALEKTQWEAKTTGTRAIKLQEQLDAVEGDISTFTRVFETLAKTD 254

Query: 195 -RAPYSRERADDSSLECEPVQLD-----DMVGDIDMEKMEQEMSAYATALAAAKDNPTDE 248
            + P       D   +  P + D     D V + D+ KME+   AY  A+  AK+   +E
Sbjct: 255 SKKP-------DRDYDAVPYEFDHLPYLDDVDETDLRKMEEARLAYVAAVNTAKEREDEE 307

Query: 249 FLKAVTEARLRLQAFVL 265
            L    +AR  LQ+   
Sbjct: 308 SLVMAAQARAYLQSLAF 324



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.309    0.124    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,265,621
Number of extensions: 147515
Number of successful extensions: 1072
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 1103
Number of HSP's successfully gapped: 3
Length of query: 265
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 168
Effective length of database: 8,447,217
Effective search space: 1419132456
Effective search space used: 1419132456
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 111 (47.4 bits)