BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os09g0456200 Os09g0456200|AK065873
(376 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G19290.3 | chr3:6687956-6689784 FORWARD LENGTH=433 124 7e-29
AT1G49720.2 | chr1:18400826-18402362 FORWARD LENGTH=404 118 4e-27
AT4G34000.1 | chr4:16296008-16297971 FORWARD LENGTH=455 117 7e-27
AT1G45249.3 | chr1:17165420-17167415 REVERSE LENGTH=428 117 1e-26
AT3G56850.1 | chr3:21046554-21047894 REVERSE LENGTH=298 89 4e-18
AT3G44460.1 | chr3:16080115-16081722 REVERSE LENGTH=332 86 4e-17
AT5G42910.1 | chr5:17203908-17205211 FORWARD LENGTH=371 84 2e-16
AT2G41070.1 | chr2:17131249-17132208 FORWARD LENGTH=263 77 1e-14
AT2G36270.1 | chr2:15204980-15206571 REVERSE LENGTH=443 65 9e-11
AT4G35900.1 | chr4:17004746-17005952 FORWARD LENGTH=286 61 1e-09
AT2G17770.2 | chr2:7723103-7723951 FORWARD LENGTH=196 56 3e-08
AT5G44080.1 | chr5:17738787-17739734 REVERSE LENGTH=316 50 2e-06
AT1G03970.1 | chr1:1018237-1019049 FORWARD LENGTH=271 49 4e-06
>AT3G19290.3 | chr3:6687956-6689784 FORWARD LENGTH=433
Length = 432
Score = 124 bits (312), Expect = 7e-29, Method: Compositional matrix adjust.
Identities = 76/151 (50%), Positives = 87/151 (57%), Gaps = 17/151 (11%)
Query: 34 LARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXXX 93
LARQ SVYSLTFDE Q+ L MNMDELL+SIWTAEE+QAM
Sbjct: 34 LARQSSVYSLTFDELQNTLGGPGKDFGS----------MNMDELLKSIWTAEEAQAMAMT 83
Query: 94 XXXXXXXXXXXXXPPT----SLQRQGSLTLPRTLSAKTVDEVWRNLVRDE---PPPVGAA 146
+LQRQGSLTLPRT+S KTVDEVW+ L+ + G
Sbjct: 84 SAPAATAVAQPGAGIPPPGGNLQRQGSLTLPRTISQKTVDEVWKCLITKDGNMEGSSGGG 143
Query: 147 DGGDMPPQRQSTLGEMTLEEFLVRAGVVREN 177
++PP RQ TLGEMTLEEFL RAGVVRE+
Sbjct: 144 GESNVPPGRQQTLGEMTLEEFLFRAGVVRED 174
Score = 89.0 bits (219), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 47/76 (61%), Positives = 59/76 (77%), Gaps = 2/76 (2%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADIMEMQKNE-VEEMIKDPF 360
MIKNRESAARSRARKQAYTLELEAE++KLK+ N+EL++KQA+++EMQKNE ++E K P+
Sbjct: 358 MIKNRESAARSRARKQAYTLELEAEIEKLKKTNQELQKKQAEMVEMQKNEQLKETSKRPW 417
Query: 361 GXXXXXXXXXXXXGPW 376
G GPW
Sbjct: 418 G-SKRQCLRRTLTGPW 432
>AT1G49720.2 | chr1:18400826-18402362 FORWARD LENGTH=404
Length = 403
Score = 118 bits (296), Expect = 4e-27, Method: Compositional matrix adjust.
Identities = 72/145 (49%), Positives = 84/145 (57%), Gaps = 14/145 (9%)
Query: 34 LARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXXX 93
LARQ S+YSLTFDE QS L MNMDELL++IWTAE++QA
Sbjct: 23 LARQSSLYSLTFDELQSTLGEPGKDFGS----------MNMDELLKNIWTAEDTQAFMTT 72
Query: 94 XXXXXXXXXXXXXPP-TSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPVGAADGGDMP 152
P LQRQGSLTLPRTLS KTVDEVW+ L E + G
Sbjct: 73 TSSVAAPGPSGFVPGGNGLQRQGSLTLPRTLSQKTVDEVWKYLNSKEGS---NGNTGTDA 129
Query: 153 PQRQSTLGEMTLEEFLVRAGVVREN 177
+RQ TLGEMTLE+FL+RAGVV+E+
Sbjct: 130 LERQQTLGEMTLEDFLLRAGVVKED 154
Score = 77.0 bits (188), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 36/51 (70%), Positives = 46/51 (90%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADIMEMQKNEV 352
MIKNRESAARSRARKQAYTLELEAE++ LK +N++L++KQA+IM+ +EV
Sbjct: 318 MIKNRESAARSRARKQAYTLELEAEIESLKLVNQDLQKKQAEIMKTHNSEV 368
>AT4G34000.1 | chr4:16296008-16297971 FORWARD LENGTH=455
Length = 454
Score = 117 bits (294), Expect = 7e-27, Method: Compositional matrix adjust.
Identities = 74/170 (43%), Positives = 88/170 (51%), Gaps = 36/170 (21%)
Query: 34 LARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXXX 93
L RQ SV+SLTFDEFQ++ MNMDELL++IWTAEES +M
Sbjct: 27 LTRQNSVFSLTFDEFQNSWGGGIGKDFGS---------MNMDELLKNIWTAEESHSMMGN 77
Query: 94 XXXXXXXXXXXXXPPT--------------------------SLQRQGSLTLPRTLSAKT 127
SLQRQGSLTLPRT+S K
Sbjct: 78 NTSYTNISNGNSGNTVINGGGNNIGGLAVGVGGESGGFFTGGSLQRQGSLTLPRTISQKR 137
Query: 128 VDEVWRNLVRDEPPPVGAADGGDMP-PQRQSTLGEMTLEEFLVRAGVVRE 176
VD+VW+ L++++ G +GG PQRQ TLGEMTLEEFLVRAGVVRE
Sbjct: 138 VDDVWKELMKEDDIGNGVVNGGTSGIPQRQQTLGEMTLEEFLVRAGVVRE 187
Score = 88.6 bits (218), Expect = 5e-18, Method: Compositional matrix adjust.
Identities = 44/76 (57%), Positives = 57/76 (75%), Gaps = 1/76 (1%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADIMEMQKNEVEEMIKDPFG 361
MIKNRESAARSRARKQAYT+ELEAE+ +LKE+N+EL++KQ +IME QKN++ E ++ P+G
Sbjct: 379 MIKNRESAARSRARKQAYTMELEAEIAQLKELNEELQKKQVEIMEKQKNQLLEPLRQPWG 438
Query: 362 XXXXXXX-XXXXXGPW 376
GPW
Sbjct: 439 MGCKRQCLRRTLTGPW 454
>AT1G45249.3 | chr1:17165420-17167415 REVERSE LENGTH=428
Length = 427
Score = 117 bits (293), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 74/150 (49%), Positives = 88/150 (58%), Gaps = 24/150 (16%)
Query: 34 LARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXXX 93
L RQGS+YSLTFDEFQS++ MNMDELL++IW+AEE+QAM
Sbjct: 21 LTRQGSIYSLTFDEFQSSVGKDFGS-------------MNMDELLKNIWSAEETQAMASG 67
Query: 94 XXXXXXXXXXXXXPPTSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPVGAAD------ 147
LQRQGSLTLPRTLS KTVD+VW++L + VG ++
Sbjct: 68 VVPVLGGGQEGLQ----LQRQGSLTLPRTLSQKTVDQVWKDLSKVGSSGVGGSNLSQVAQ 123
Query: 148 -GGDMPPQRQSTLGEMTLEEFLVRAGVVRE 176
QRQ TLGE+TLEEFLVRAGVVRE
Sbjct: 124 AQSQSQSQRQQTLGEVTLEEFLVRAGVVRE 153
Score = 79.7 bits (195), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 54/105 (51%), Positives = 57/105 (54%), Gaps = 13/105 (12%)
Query: 262 KGNSDLSSPTEPMPYSFXXXXXXXXXXXXXXXXXXXXXXXMIKNRESAARSRARKQ---- 317
K N D SS P PY F MIKNRESAARSRARKQ
Sbjct: 305 KSNGD-SSSLSPSPYMFNGGVRGRKSGTVEKVVERRQRR-MIKNRESAARSRARKQIFTA 362
Query: 318 -------AYTLELEAEVQKLKEMNKELERKQADIMEMQKNEVEEM 355
AYT+ELEAEV KLKE N EL+RKQA IMEMQKN+ EM
Sbjct: 363 DQTIYWNAYTVELEAEVAKLKEENDELQRKQARIMEMQKNQETEM 407
>AT3G56850.1 | chr3:21046554-21047894 REVERSE LENGTH=298
Length = 297
Score = 89.0 bits (219), Expect = 4e-18, Method: Compositional matrix adjust.
Identities = 58/146 (39%), Positives = 77/146 (52%), Gaps = 26/146 (17%)
Query: 33 TLARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXX 92
+L RQ S+YSLT DE Q+ L MN+DELL+S+ + E +Q
Sbjct: 15 SLNRQSSLYSLTLDEVQNHLGSSGKALGS----------MNLDELLKSVCSVEANQPSSM 64
Query: 93 XXXXXXXXXXXXXXPPTSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPVGAADGGDMP 152
L RQGSLTLPR LS KTVDEVW+++ +++ +GG
Sbjct: 65 AVNGGAAAQE-------GLSRQGSLTLPRDLSKKTVDEVWKDIQQNK-------NGGSAH 110
Query: 153 PQR--QSTLGEMTLEEFLVRAGVVRE 176
+R Q TLGEMTLE+ L++AGVV E
Sbjct: 111 ERRDKQPTLGEMTLEDLLLKAGVVTE 136
Score = 57.0 bits (136), Expect = 2e-08, Method: Compositional matrix adjust.
Identities = 30/43 (69%), Positives = 36/43 (83%), Gaps = 1/43 (2%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADI 344
MIKNRESAARSRARKQAYT ELE +V +L+E N+ L RKQ ++
Sbjct: 232 MIKNRESAARSRARKQAYTHELEIKVSRLEEENERL-RKQKEV 273
>AT3G44460.1 | chr3:16080115-16081722 REVERSE LENGTH=332
Length = 331
Score = 85.5 bits (210), Expect = 4e-17, Method: Compositional matrix adjust.
Identities = 56/146 (38%), Positives = 69/146 (47%), Gaps = 22/146 (15%)
Query: 34 LARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXXX 93
+ RQ S+ SLT DE Q MNMDE L ++WT E
Sbjct: 35 VGRQNSILSLTLDEIQ-------------MKSGKSFGAMNMDEFLANLWTTVEEN----- 76
Query: 94 XXXXXXXXXXXXXPPTSLQRQGSLTLPRTLSAKTVDEVW---RNLVRDEPPPVGAADGGD 150
P L RQGSL+LP L KTVDEVW +N V+ PP +
Sbjct: 77 -DNEGGGAHNDGEKPAVLPRQGSLSLPVPLCKKTVDEVWLEIQNGVQQHPPSSNSGQNSA 135
Query: 151 MPPQRQSTLGEMTLEEFLVRAGVVRE 176
+RQ TLGE+TLE+FLV+AGVV+E
Sbjct: 136 ENIRRQQTLGEITLEDFLVKAGVVQE 161
>AT5G42910.1 | chr5:17203908-17205211 FORWARD LENGTH=371
Length = 370
Score = 83.6 bits (205), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 52/140 (37%), Positives = 70/140 (50%), Gaps = 33/140 (23%)
Query: 33 TLARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXX 92
+L+RQGS+YS T D+FQ++L MNMDEL++ I +AEE+Q
Sbjct: 19 SLSRQGSIYSWTVDQFQTSLGLDCGS-------------MNMDELVKHISSAEETQE--- 62
Query: 93 XXXXXXXXXXXXXXPPTSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPVGAADGGDMP 152
QRQGS TLP TLS + V EVW+++ ++
Sbjct: 63 -----------------GSQRQGSTTLPPTLSKQNVGEVWKSITEEKHTNNNGGVTNITH 105
Query: 153 PQRQSTLGEMTLEEFLVRAG 172
Q Q TLGE+TLEEF +RAG
Sbjct: 106 LQGQQTLGEITLEEFFIRAG 125
Score = 50.4 bits (119), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 25/46 (54%), Positives = 36/46 (78%)
Query: 303 IKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADIMEMQ 348
IKNRESAARSRARKQA T+E+E E++ LK+ +EL ++ ++ + Q
Sbjct: 301 IKNRESAARSRARKQAQTMEVEVELENLKKDYEELLKQHVELRKRQ 346
>AT2G41070.1 | chr2:17131249-17132208 FORWARD LENGTH=263
Length = 262
Score = 77.4 bits (189), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 53/144 (36%), Positives = 68/144 (47%), Gaps = 36/144 (25%)
Query: 33 TLARQGSVYSLTFDEFQSALAXXXXXXXXXXXXXXXXXXMNMDELLRSIWTAEESQAMXX 92
+L RQ S+YSL E Q+ L MN+DELL+++ E
Sbjct: 15 SLTRQNSLYSLKLHEVQTHLGSSGKPLGS----------MNLDELLKTVLPPAEE----- 59
Query: 93 XXXXXXXXXXXXXXPPTSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPVGAADGGDMP 152
L RQGSLTLPR LS KTVDEVWR++ +D+
Sbjct: 60 -----------------GLVRQGSLTLPRDLSKKTVDEVWRDIQQDK----NGNGTSTTT 98
Query: 153 PQRQSTLGEMTLEEFLVRAGVVRE 176
+Q TLGE+TLE+ L+RAGVV E
Sbjct: 99 THKQPTLGEITLEDLLLRAGVVTE 122
Score = 55.8 bits (133), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 28/38 (73%), Positives = 33/38 (86%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELER 339
MIKNRESAARSRARKQAYT ELE +V +L+E N++L R
Sbjct: 197 MIKNRESAARSRARKQAYTHELEIKVSRLEEENEKLRR 234
>AT2G36270.1 | chr2:15204980-15206571 REVERSE LENGTH=443
Length = 442
Score = 64.7 bits (156), Expect = 9e-11, Method: Compositional matrix adjust.
Identities = 40/84 (47%), Positives = 47/84 (55%), Gaps = 16/84 (19%)
Query: 109 TSLQRQGSLTLPRTLSAKTVDEVWRNLVRDEPPPV---------------GAADGGDMPP 153
+SL RQGSLTLP L KTVDEVW + R A +GG+
Sbjct: 138 SSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAA 197
Query: 154 QRQSTLGEMTLEEFLVRAGVVREN 177
RQ T GEMTLE+FLV+AGVVRE+
Sbjct: 198 -RQPTFGEMTLEDFLVKAGVVREH 220
Score = 64.3 bits (155), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 33/56 (58%), Positives = 43/56 (76%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQADIMEMQKNEVEEMIK 357
MIKNRESAARSRARKQAYT+ELEAE+ +LKE N +L+ A++ +K + E +K
Sbjct: 362 MIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLK 417
>AT4G35900.1 | chr4:17004746-17005952 FORWARD LENGTH=286
Length = 285
Score = 60.8 bits (146), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 35/65 (53%), Positives = 42/65 (64%), Gaps = 6/65 (9%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKELERKQ------ADIMEMQKNEVEEM 355
MIKNRESAARSRARKQAYT ELE EV L+ N L+R+Q A I + +KN ++
Sbjct: 221 MIKNRESAARSRARKQAYTNELELEVAHLQAENARLKRQQDQLKMAAAIQQPKKNTLQRS 280
Query: 356 IKDPF 360
PF
Sbjct: 281 STAPF 285
>AT2G17770.2 | chr2:7723103-7723951 FORWARD LENGTH=196
Length = 195
Score = 55.8 bits (133), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 31/58 (53%), Positives = 42/58 (72%), Gaps = 2/58 (3%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKEL--ERKQADIMEMQKNEVEEMIK 357
MIKNRESAARSRARKQAYT ELE E+ L+ N L +++Q I E +N+V++ ++
Sbjct: 131 MIKNRESAARSRARKQAYTNELELEIAHLQTENARLKIQQEQLKIAEATQNQVKKTLQ 188
>AT5G44080.1 | chr5:17738787-17739734 REVERSE LENGTH=316
Length = 315
Score = 50.4 bits (119), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 29/44 (65%), Positives = 33/44 (75%), Gaps = 4/44 (9%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMN----KELERKQ 341
MIKNRESAARSR RKQAY +ELEA KL+E N KE+E K+
Sbjct: 238 MIKNRESAARSRERKQAYQVELEALAAKLEEENELLSKEIEDKR 281
>AT1G03970.1 | chr1:1018237-1019049 FORWARD LENGTH=271
Length = 270
Score = 48.9 bits (115), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 25/36 (69%), Positives = 29/36 (80%)
Query: 302 MIKNRESAARSRARKQAYTLELEAEVQKLKEMNKEL 337
MIKNRESAARSR RKQAY +ELE KL+E N++L
Sbjct: 194 MIKNRESAARSRERKQAYQVELETLAAKLEEENEQL 229
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.129 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,669,504
Number of extensions: 135192
Number of successful extensions: 712
Number of sequences better than 1.0e-05: 13
Number of HSP's gapped: 689
Number of HSP's successfully gapped: 23
Length of query: 376
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 276
Effective length of database: 8,364,969
Effective search space: 2308731444
Effective search space used: 2308731444
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 112 (47.8 bits)