BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0440700 Os09g0440700|AK104963
         (149 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G20650.1  | chr5:6985481-6985921 REVERSE LENGTH=147             93   4e-20

>AT5G20650.1 | chr5:6985481-6985921 REVERSE LENGTH=147
          Length = 146

 Score = 93.2 bits (230), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 56/149 (37%), Positives = 64/149 (42%), Gaps = 3/149 (2%)

Query: 1   MMHMTFYWGKDVTILFDGWRTATWTGYXXXXXXXXXXXXFYQYLEAFRIRVKLLXXXXXX 60
           MMHMTFYWG   TILFD W+T +W  Y            FYQYLE  RI+ K L      
Sbjct: 1   MMHMTFYWGIKATILFDFWKTDSWLSYILTLIACFVFSAFYQYLENRRIQFKSLSSSRRA 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXGRWPARLATAGLFGVNSGLGYLLMLAVMSFNXXXXXXX 120
                                  R  A+ A+  LFGVN+ +GYLLMLA MSFN       
Sbjct: 61  PPPPRSSSGVSAPLIPKSGT---RSAAKAASVLLFGVNAAIGYLLMLAAMSFNGGVFIAI 117

Query: 121 XXXXXXXXXXXRSSDGEDLVVVDNPCACA 149
                      RS DG      D+PC CA
Sbjct: 118 VVGLTAGYAVFRSDDGGADTATDDPCPCA 146



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.329    0.142    0.481 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,696,891
Number of extensions: 29740
Number of successful extensions: 84
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 2
Length of query: 149
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 60
Effective length of database: 8,666,545
Effective search space: 519992700
Effective search space used: 519992700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 107 (45.8 bits)