BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0421500 Os09g0421500|AK059873
         (198 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G57910.1  | chr5:23451259-23452463 REVERSE LENGTH=208           54   6e-08

>AT5G57910.1 | chr5:23451259-23452463 REVERSE LENGTH=208
          Length = 207

 Score = 53.5 bits (127), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 48/171 (28%), Positives = 85/171 (49%), Gaps = 30/171 (17%)

Query: 50  DNEMKRRKLESINKRKLVLLSEVKFLQKKLNSFKKNDSQQVRLKK-----KAPRV---PS 101
           + E  R++L+++ +RK  L++EV+FL+++    +++  Q+++  +     K  RV   PS
Sbjct: 38  ETEAMRKRLQAVRERKATLMAEVRFLRRRYRHLRQDQPQEIKKVRRSNGGKKIRVEVSPS 97

Query: 102 --------HVGINDASAFYGASTEVPSTSKRTD--LDLNQDSAMNDELSDFPGHHNHLEL 151
                   HV + D +    A  E  ++ KR     DLNQ S   +E  +     N+ E 
Sbjct: 98  NKSEAETKHVSLPDLNHSEKAHDETKTSPKRRVPLFDLNQISG--EEEQETEAVDNNQEN 155

Query: 152 KKAEQAGVDEDIMTA-------DVNLSACRDTGNSPASDDKRSVSWQDRVA 195
            + E++   + +M +       DV  S+CR+ GN     +KR +SWQD VA
Sbjct: 156 TRVEESNSCKRLMISSIEMQQKDVKPSSCRNGGN---GSNKRKISWQDPVA 203



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.127    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,468,831
Number of extensions: 130512
Number of successful extensions: 385
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 384
Number of HSP's successfully gapped: 1
Length of query: 198
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 105
Effective length of database: 8,556,881
Effective search space: 898472505
Effective search space used: 898472505
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 109 (46.6 bits)