BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0421200 Os09g0421200|AK059290
         (336 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G10180.1  | chr3:3146393-3154644 REVERSE LENGTH=1274           122   2e-28

>AT3G10180.1 | chr3:3146393-3154644 REVERSE LENGTH=1274
          Length = 1273

 Score =  122 bits (307), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 92/280 (32%), Positives = 151/280 (53%), Gaps = 7/280 (2%)

Query: 2    EKKILDEQSLDQKDELQRLKSSLESCEKAMEDCNIQNELEKDSILSELLTLQKEVVYXXX 61
            EK +L EQ    + +++ L    +  E +++  +  +E E+  +LS +  L+K++     
Sbjct: 765  EKLLLQEQCAGLQSQIEELNQEAQKHETSLKMLSEHHESERSDLLSHIECLEKDIGSLSS 824

Query: 62   XXXXXXXXXIRKELDRXXXXXXXXXXXXXXSIQEKIKLESEKAEAQREIKKLQSQRTLLE 121
                     +RK+ ++              S+Q+K KLE+EKA A+RE+K+L SQ+ LLE
Sbjct: 825  SSLAKEKENLRKDFEKTKTKLKDTESKLKNSMQDKTKLEAEKASAERELKRLHSQKALLE 884

Query: 122  RDLRKRDSFTVDKRHEQSVKSKELAGIYDQAVQIQEDYGKLEMHAFDMEAEIASLQEALV 181
            RD+ K++SF   +R    V+        +Q+  +QE++ +LE+ AF+ME  IASL+E L 
Sbjct: 885  RDISKQESFAGKRRDSLLVERS-----ANQS--LQEEFKQLEVLAFEMETTIASLEEELA 937

Query: 182  TTIAEKEEALSRVELLTSAVEDLESRLNXXXXXXXXXXXXXXVLTRKLDASESISKKLEA 241
                EKEEAL R + L S + DL  +L                L  +L+ S S  ++LE 
Sbjct: 938  AERGEKEEALCRNDGLGSEITDLTEKLEHSNTKLEHLQNDVTELKTRLEVSSSDQQQLET 997

Query: 242  SISSLSREKEDMGIELTDVLLEMESERSTWTAKEKAYLEA 281
            ++  L  EKE++ + L + LLEME E++ W++KEKA  EA
Sbjct: 998  NVKQLLEEKEELAMHLANSLLEMEEEKAIWSSKEKALTEA 1037



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.309    0.125    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,995,713
Number of extensions: 161834
Number of successful extensions: 1031
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1040
Number of HSP's successfully gapped: 2
Length of query: 336
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 237
Effective length of database: 8,392,385
Effective search space: 1988995245
Effective search space used: 1988995245
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 112 (47.8 bits)