BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0394100 Os09g0394100|AK120537
         (514 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G04320.1  | chr4:2113565-2116525 FORWARD LENGTH=519            558   e-159

>AT4G04320.1 | chr4:2113565-2116525 FORWARD LENGTH=519
          Length = 518

 Score =  558 bits (1439), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 275/461 (59%), Positives = 341/461 (73%), Gaps = 18/461 (3%)

Query: 52  RHWLHASVSSSASTALD----RFSDGYRSLDRPGRREILRSLAADYDVPRARVRDLMRQY 107
           R  +H+++S+S +  LD     F +GY SL    R ++L  LA +YDV R +VR+L++QY
Sbjct: 62  RETMHSAISASKTGVLDITLNDFQEGYFSLSLEDREKLLLVLAKEYDVNREQVRELVKQY 121

Query: 108 LXXXXXXXXXXXXXXXXXXXXXXXXXXMYRMERGLREALRPKYAGFLEAMNAQPGGLKLL 167
           L                           YR+ER LR ALRP Y    E +N  PGGL+ L
Sbjct: 122 LGLETPASDDDKGALSSV---------FYRIERNLRHALRPTYEVLFERLNTHPGGLRFL 172

Query: 168 AVIRADLLALLGEENLPALRALDGYLKEKLVTWLSPAALTLHQITWDDPASLLEKIVAYE 227
           +++RADLL++L +EN P+LR LD +LKEKL  WLSPA L LHQITWDDPASLLEKIVAYE
Sbjct: 173 SILRADLLSILSKENTPSLRTLDSFLKEKLGMWLSPATLELHQITWDDPASLLEKIVAYE 232

Query: 228 AVHPIRNLIDLKRRLGVGRRCFGYFHPAIPGEPLIFIEVALLKDTAASIQEVLWDDPPTP 287
           AVHPI NL+DLKRRLG+GRRCFGYFHP++PGEPLIFIEVAL++  A +IQEVLWD+PP P
Sbjct: 233 AVHPISNLLDLKRRLGIGRRCFGYFHPSVPGEPLIFIEVALMETVAQTIQEVLWDNPPIP 292

Query: 288 ESEARCALFYSISSTQPGLSGINLGKFLLKRVIEMLRRDMPSVQIFATLSPIPGFMQWLL 347
           E++A CALFYSISSTQPGL+GINLGKFL+KRVI ++++DMP V  FATLSPIPGFMQWLL
Sbjct: 293 ENQATCALFYSISSTQPGLAGINLGKFLIKRVITLVKKDMPHVSTFATLSPIPGFMQWLL 352

Query: 348 AKLASQIKLAEAESQDGSLLEGTSSTFRESILFPEEERMIHD-AVEHAGGKSGIKLLQDI 406
           +KL+SQ + AE E   G+     SSTF E +L PEEE  +   + E + G +G+++L ++
Sbjct: 353 SKLSSQSRFAEDER--GTQSNSPSSTFSEKVLLPEEEHALMTLSDESSSGSNGMEVLLNL 410

Query: 407 L--KSSQWVKSDKLSSALKSPLMRLCARYLAREKKRGKALDAVANFHLQNGAMIERINWM 464
           L  K+  W  S +L   L+  LMRLCARYL +EKKRGKALD+VANFHLQNGAM+ERINWM
Sbjct: 411 LSVKNCDWATSPRLLPVLEPILMRLCARYLLQEKKRGKALDSVANFHLQNGAMVERINWM 470

Query: 465 ADQSEKGIQQSGGIMVNYMYRLENIEEYALSYLGTGLAHTS 505
           AD+SEKGI+QSGGIMVNY+YRLENIE+YA SY G+G  H S
Sbjct: 471 ADRSEKGIRQSGGIMVNYVYRLENIEDYAQSYFGSGKIHAS 511



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,454,411
Number of extensions: 359294
Number of successful extensions: 834
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 830
Number of HSP's successfully gapped: 1
Length of query: 514
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 411
Effective length of database: 8,282,721
Effective search space: 3404198331
Effective search space used: 3404198331
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 114 (48.5 bits)