BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0318600 Os09g0318600|AK101412
         (527 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G41220.1  | chr5:16494560-16496969 REVERSE LENGTH=591           53   5e-07
AT5G41240.1  | chr5:16498293-16500811 REVERSE LENGTH=592           49   7e-06

>AT5G41220.1 | chr5:16494560-16496969 REVERSE LENGTH=591
          Length = 590

 Score = 52.8 bits (125), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 20/133 (15%)

Query: 125 RLYWTEKEDIRLISAWLIY---------YKTDRYWEKVVAEYN--STIPATRRRELQHVK 173
           R  W+  ED  LISAWL           +K   +W+++ A +N  +++    +RE  H K
Sbjct: 270 RRKWSRAEDAILISAWLNTSKDPIVDNEHKACAFWKRIGAYFNNSASLANLPKREPSHCK 329

Query: 174 GHWHKIFRKVAHFHDCWCRVKAKYPSGHSEGMQLMDKTWLMYNEEARVMYLEEAEHNFAF 233
             W K+  KV  F  C+ +   +  SG SE     D   + Y      +Y    + NF  
Sbjct: 330 QRWSKLNDKVCKFVGCYDQALNQRSSGQSED----DVFQVAYQ-----VYTNNYKSNFTL 380

Query: 234 DHCWKAVWNQPKW 246
           +H W+ + +  KW
Sbjct: 381 EHAWRELRHSKKW 393



>AT5G41240.1 | chr5:16498293-16500811 REVERSE LENGTH=592
          Length = 591

 Score = 48.9 bits (115), Expect = 7e-06,   Method: Compositional matrix adjust.
 Identities = 35/140 (25%), Positives = 61/140 (43%), Gaps = 22/140 (15%)

Query: 120 EKATKRLYWTEKEDIRLISAWL--------IYY---KTDRYWEKVVAEYN--STIPATRR 166
           ++  +R  W+  +D+ LISAWL        + Y   +   +W+++ A  +  +++    +
Sbjct: 265 DRKARRRKWSPPDDVILISAWLNTSKDRKVVVYDEQQAHTFWKRIGAHVSNSASLANLPK 324

Query: 167 RELQHVKGHWHKIFRKVAHFHDCWCRVKAKYPSGHSEGMQLMDKTWLMYNEEARVMYLEE 226
           RE  H +  W KI   V  F  C+ +   +  SG SE         L YN      Y+  
Sbjct: 325 REWNHCRQRWRKINDYVCKFVGCYDQALNQRASGQSEDDVFQVAYQLYYNN-----YMS- 378

Query: 227 AEHNFAFDHCWKAVWNQPKW 246
              NF  +H W+ + +  KW
Sbjct: 379 ---NFKLEHAWRELRHNKKW 395



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.129    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 10,336,777
Number of extensions: 395980
Number of successful extensions: 1203
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1201
Number of HSP's successfully gapped: 2
Length of query: 527
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 424
Effective length of database: 8,282,721
Effective search space: 3511873704
Effective search space used: 3511873704
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 114 (48.5 bits)