BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0561600 Os08g0561600|AK066884
(577 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G28150.1 | chr2:12000015-12002227 FORWARD LENGTH=541 180 2e-45
AT5G59790.1 | chr5:24090950-24092804 FORWARD LENGTH=424 169 3e-42
AT3G46110.1 | chr3:16932244-16933588 FORWARD LENGTH=344 146 3e-35
AT5G10150.1 | chr5:3182176-3183834 REVERSE LENGTH=415 132 5e-31
AT1G05577.1 | chr1:1662643-1664288 REVERSE LENGTH=373 109 5e-24
>AT2G28150.1 | chr2:12000015-12002227 FORWARD LENGTH=541
Length = 540
Score = 180 bits (457), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 83/128 (64%), Positives = 95/128 (74%), Gaps = 12/128 (9%)
Query: 16 SPGRNKVWVEPPGKTHHLRXXXXXXXXXXXKRVAVVYYLCRNRHLEHPHFIEVPLASPDG 75
SP R KVW E K H K+V +VYYL +NR LEHPHF+EV ++SP+G
Sbjct: 13 SPERAKVWTEKSPKYHQ-----------KIKKVQIVYYLSKNRQLEHPHFMEVLISSPNG 61
Query: 76 LYLRDVINRLNVLRGKGMAAMYSWSCKSRSYKNGFVWHDLSEDDLVLPAQGNEYILKGSE 135
LYLRDVI RLNVLRG+GMA+MYSWS K RSY+NGFVWHDLSEDDL+LPA GNEY+LKGSE
Sbjct: 62 LYLRDVIERLNVLRGRGMASMYSWSSK-RSYRNGFVWHDLSEDDLILPANGNEYVLKGSE 120
Query: 136 LLDRSPPD 143
L D S D
Sbjct: 121 LFDESNSD 128
Score = 123 bits (308), Expect = 3e-28, Method: Compositional matrix adjust.
Identities = 102/267 (38%), Positives = 141/267 (52%), Gaps = 21/267 (7%)
Query: 216 EDDQCRTP--RSGSSGNMSPEPAGRVAPPLSEASSPGPLEYRVCKPIGAQDASTQTDD-- 271
EDD+ P RS SS +SP+ + +SS EY+V K G DASTQTD+
Sbjct: 175 EDDELSPPALRSVSSSGVSPD-----SRDAKNSSSWCLAEYKVYKSEGLADASTQTDETV 229
Query: 272 SERDAPERNSRMAGVCT-ENSTSDSEIQE--------CHPRSTQPSPKGPGVVRESPPVC 322
S R + GV T E+ +S+ E E C + + + V SPP
Sbjct: 230 SGRSKTPIETFSRGVSTDEDVSSEPETSENNLVSEASCAGKERESAEISRNSV--SPPFS 287
Query: 323 SSDASPGGRVETLESLIXXXXXXXXXXXXXXXXHLFAPMGVKFKPANLLMQLITCGSISV 382
+S +S GG+ +TLESLI + P + + +N+LMQLI+CGSISV
Sbjct: 288 NSASSLGGKTDTLESLIRADVSKMNSFRILEQEDVRMPAIPRLRASNMLMQLISCGSISV 347
Query: 383 KDHRSFGLIPTYRPRFTQVEFPSPMFSTPLALRHLDNIPCNARTIGMRIPESEYFSGSLV 442
KD+ +FGL+PTY+P+F +FPSP FS+ + LD + +G+R+ E EYFSGSLV
Sbjct: 348 KDN-NFGLVPTYKPKFGHSKFPSPFFSSSFMMGDLDRLSETPSLMGLRMEEKEYFSGSLV 406
Query: 443 ETKKQDESGKGGTPTLKRSSSCSEDRV 469
ETK Q + +LKRSSS + DR
Sbjct: 407 ETKLQKKDAADSNASLKRSSSYNGDRA 433
>AT5G59790.1 | chr5:24090950-24092804 FORWARD LENGTH=424
Length = 423
Score = 169 bits (429), Expect = 3e-42, Method: Compositional matrix adjust.
Identities = 79/123 (64%), Positives = 91/123 (73%), Gaps = 13/123 (10%)
Query: 16 SPGRNKVWVEPPGKTHHLRXXXXXXXXXXXKRVAVVYYLCRNRHLEHPHFIEVPLASPDG 75
SP RN++W EP K ++V VVYYLCRN L+HPHFIEV L+S DG
Sbjct: 27 SPDRNRIWSEPRLK------------PVVNRKVPVVYYLCRNGQLDHPHFIEVTLSSHDG 74
Query: 76 LYLRDVINRLNVLRGKGMAAMYSWSCKSRSYKNGFVWHDLSEDDLVLPAQGNEYILKGSE 135
LYL+DVINRLN LRGKGMA++YSWS K RSYKNGFVWHDLSEDD + P QG EY+LKGSE
Sbjct: 75 LYLKDVINRLNDLRGKGMASLYSWSSK-RSYKNGFVWHDLSEDDFIFPVQGQEYVLKGSE 133
Query: 136 LLD 138
+LD
Sbjct: 134 VLD 136
>AT3G46110.1 | chr3:16932244-16933588 FORWARD LENGTH=344
Length = 343
Score = 146 bits (369), Expect = 3e-35, Method: Compositional matrix adjust.
Identities = 63/91 (69%), Positives = 76/91 (83%), Gaps = 1/91 (1%)
Query: 48 VAVVYYLCRNRHLEHPHFIEVPLASPDGLYLRDVINRLNVLRGKGMAAMYSWSCKSRSYK 107
V VVYYL RN L+HPHFIEVPL+S +GLYL+DVINRLN LRG GMA +YSWS K R+YK
Sbjct: 20 VPVVYYLSRNGRLDHPHFIEVPLSSHNGLYLKDVINRLNDLRGNGMACLYSWSSK-RTYK 78
Query: 108 NGFVWHDLSEDDLVLPAQGNEYILKGSELLD 138
NGFVW+DLS++D + P G EY+LKGS++LD
Sbjct: 79 NGFVWYDLSDEDFIFPVHGQEYVLKGSQILD 109
>AT5G10150.1 | chr5:3182176-3183834 REVERSE LENGTH=415
Length = 414
Score = 132 bits (332), Expect = 5e-31, Method: Compositional matrix adjust.
Identities = 60/96 (62%), Positives = 76/96 (79%), Gaps = 5/96 (5%)
Query: 46 KRVAVVYYLCRNRHLEHPHFIEV--PLASPDGLYLRDVINRLNVLRGKGMAAMYSWSCKS 103
+RV VVYYL RN HLEHPHFIEV P+ P L LRDV+NRL +LRGK M + Y+WSCK
Sbjct: 44 RRVQVVYYLTRNGHLEHPHFIEVISPVNQP--LRLRDVMNRLTILRGKCMTSQYAWSCK- 100
Query: 104 RSYKNGFVWHDLSEDDLVLPAQGNEYILKGSELLDR 139
RSY+NGFVW+DL+E+D++ P+ EY+LKGSE+ D+
Sbjct: 101 RSYRNGFVWNDLAENDVIYPSDCAEYVLKGSEITDK 136
>AT1G05577.1 | chr1:1662643-1664288 REVERSE LENGTH=373
Length = 372
Score = 109 bits (272), Expect = 5e-24, Method: Compositional matrix adjust.
Identities = 50/98 (51%), Positives = 67/98 (68%), Gaps = 1/98 (1%)
Query: 46 KRVAVVYYLCRNRHLEHPHFIEVPLASPDGLYLRDVINRLNVLRGKGMAAMYSWSCKSRS 105
+RV +VY+L R+ H++HPH + V S +G++LRDV L RG M +SWSCK R
Sbjct: 11 RRVNLVYFLSRSGHVDHPHLLRVHHLSRNGVFLRDVKKWLADARGDAMPDAFSWSCK-RR 69
Query: 106 YKNGFVWHDLSEDDLVLPAQGNEYILKGSELLDRSPPD 143
YKNG+VW DL +DDL+ P NEY+LKGSE+L SP +
Sbjct: 70 YKNGYVWQDLLDDDLITPISDNEYVLKGSEILLSSPKE 107
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.130 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,515,618
Number of extensions: 486658
Number of successful extensions: 1195
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 1185
Number of HSP's successfully gapped: 6
Length of query: 577
Length of database: 11,106,569
Length adjustment: 104
Effective length of query: 473
Effective length of database: 8,255,305
Effective search space: 3904759265
Effective search space used: 3904759265
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 114 (48.5 bits)