BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0536300 Os08g0536300|AK099722
         (488 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G33500.1  | chr2:14188159-14190046 REVERSE LENGTH=403           99   4e-21
AT1G28050.1  | chr1:9775768-9777657 REVERSE LENGTH=434             81   2e-15

>AT2G33500.1 | chr2:14188159-14190046 REVERSE LENGTH=403
          Length = 402

 Score = 99.4 bits (246), Expect = 4e-21,   Method: Compositional matrix adjust.
 Identities = 117/463 (25%), Positives = 171/463 (36%), Gaps = 78/463 (16%)

Query: 18  CDYCGEXXXXXXXXXXXXXXXVACDRHVHAANALSRKHXXXXXXXXXXXXXXXXXXXXXX 77
           C++CGE               + CD+HVH+AN LSRKH                      
Sbjct: 12  CEFCGERTAVLFCRADTAKLCLPCDQHVHSANLLSRKHVRSQICDNCSKEPVSVRCFTDN 71

Query: 78  XXXFLXXX-XXXXXXXXXXXXLRVPVEGFSGCPAAAELAASWGLDLPXXXXXXXXXADDA 136
                                 R  VEGFSGCP+  ELAA WG+DL              
Sbjct: 72  LVLCQECDWDVHGSCSSSATHERSAVEGFSGCPSVLELAAVWGIDLKGKKKEDDEDELTK 131

Query: 137 FFSALDYSMLAVDPVLRDLYVPCDPPEVVVAGGGRRLKGEALGHQLAEMARREAETAHPH 196
            F     S  +   ++++L VP D      +    R K + +  QL  + R   E     
Sbjct: 132 NFGMGLDSWGSGSNIVQELIVPYDVSCKKQSFSFGRSK-QVVFEQLELLKRGFVE----- 185

Query: 197 TQPHSDLSPRTPRRTSAAASGRLQEKQXXXXXXXXXXXXXXXXYTSLLMMAPANCTELME 256
                               G +   +                +TSLLM + + C   M+
Sbjct: 186 ------------------GEGEIMVPEGINGGGSISQPSPTTSFTSLLM-SQSLCGNGMQ 226

Query: 257 NNRVGDEDENVLWESTAPSV-PPTQIWDFNLGKSRDHNENSALEVGFGSNNGGFMIKSYN 315
                       W +T  S    TQIWDFNLG+SR+ +E S +E                
Sbjct: 227 ------------WNATNHSTGQNTQIWDFNLGQSRNPDEPSPVE---------------- 258

Query: 316 DMLKEISSGTTKDLEDIYDSRYFAAAEDIMSTNVCQLSSKNPSTRSNKRKASSCASTIDG 375
                 + G+T    ++   +      D  +TN+      N    S +++ S  +++  G
Sbjct: 259 ------TKGSTFTFNNVTHLK-----NDTRTTNM------NAFKESYQQEDSVHSTSTKG 301

Query: 376 PTTSTSH-VPAASGALGGSSNDRGSALPKEISFCDQTVVPTGADQRPCTIKIDSETLAQN 434
             TS S+ +PAA  +   S++  G    + I+     +    A +       D E +AQN
Sbjct: 302 QETSKSNNIPAAIHSHKSSNDSCGLHCTEHIA-----ITSNRATRLVAVTNADLEQMAQN 356

Query: 435 RDSAMQXXXXXXXXXXXXXHIRYESRKLRADTRKRVKGRFVKS 477
           RD+AMQ              IRYE+RK RA+TR RVKGRFVK+
Sbjct: 357 RDNAMQRYKEKKKTRRYDKTIRYETRKARAETRLRVKGRFVKA 399



>AT1G28050.1 | chr1:9775768-9777657 REVERSE LENGTH=434
          Length = 433

 Score = 80.9 bits (198), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 65/206 (31%), Positives = 87/206 (42%), Gaps = 38/206 (18%)

Query: 279 TQIWDFNLGKSRDHNENSALEVGFGSNNGG--FMIKSYNDMLKEISSGTTKDLEDIYDSR 336
           +QIWDFNLG+SR   + S +E  +        F I ++ D + E  S   K +++I    
Sbjct: 261 SQIWDFNLGQSRGPEDTSRVEAAYVGKGAASSFTINNFVDHMNETCSTNVKGVKEIKKDD 320

Query: 337 YFAAAEDIMSTNVCQLSSKNPSTRSNKRKASSCASTIDGPTTSTSH-VPAASGALGGSSN 395
           Y                         KR  S        PT S S+  P   G+      
Sbjct: 321 Y-------------------------KRSTSGQVQ----PTKSESNNRPITFGS------ 345

Query: 396 DRGSALPKEISFCDQTVVPTGADQRPCTIKIDSETLAQNRDSAMQXXXXXXXXXXXXXHI 455
           ++GS    ++ F +     +    R    K D E LAQNR  AMQ              I
Sbjct: 346 EKGSNSSSDLHFTEHIAGTSCKTTRLVATKADLERLAQNRGDAMQRYKEKRKTRRYDKTI 405

Query: 456 RYESRKLRADTRKRVKGRFVKSNGAP 481
           RYESRK RADTR RV+GRFVK++ AP
Sbjct: 406 RYESRKARADTRLRVRGRFVKASEAP 431



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.129    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,473,875
Number of extensions: 314193
Number of successful extensions: 719
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 722
Number of HSP's successfully gapped: 2
Length of query: 488
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 386
Effective length of database: 8,310,137
Effective search space: 3207712882
Effective search space used: 3207712882
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 114 (48.5 bits)