BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0534900 Os08g0534900|AK061777
(346 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G14790.1 | chr5:4784058-4785508 FORWARD LENGTH=347 327 6e-90
AT3G01450.1 | chr3:169903-171379 REVERSE LENGTH=327 301 2e-82
AT4G15830.1 | chr4:8995285-8996548 REVERSE LENGTH=297 207 9e-54
AT3G18530.1 | chr3:6373535-6375050 REVERSE LENGTH=362 155 4e-38
>AT5G14790.1 | chr5:4784058-4785508 FORWARD LENGTH=347
Length = 346
Score = 327 bits (838), Expect = 6e-90, Method: Compositional matrix adjust.
Identities = 186/354 (52%), Positives = 243/354 (68%), Gaps = 29/354 (8%)
Query: 1 MSDSALKDLNLAQSAELEKTKDSSAKSCITKPVLNGNKCNNTEENAPPVLPDAVTNGC-- 58
M+ + LK++N E K S K+ +TKP + K N E+ P LP++ +
Sbjct: 1 MASNTLKEMNSLHVTE----KISDCKASLTKPCVG--KMNGKSEDRP--LPNSASLDSSD 52
Query: 59 -----------EAGNADVEYIDSESLTDLEDAGATLSTLVARLDSKDWVMTCEALNNVRQ 107
E +VEYI+SE+L +++DA A L +++A L+SKDW+ C+ALNNVR+
Sbjct: 53 SKVVEAEKPEPEIAIVEVEYIESENLDNVDDADAVLKSVLAGLESKDWISLCDALNNVRR 112
Query: 108 LAIFHKDRLQELLEPLVPLIVKSVKNPRSAVCKTALMTCADIFKAYGDLMVDSIDPLLVQ 167
L+IFHK+ + +LE ++PL+VKS+KNPRSAVCKTA MT ADIF AY + + D ++PLL Q
Sbjct: 113 LSIFHKEEMMHMLEKVIPLVVKSLKNPRSAVCKTACMTSADIFSAYNNHITDLLEPLLTQ 172
Query: 168 LFLKSSQDKRFVCEAAEAALISMTSWIAPSALLPKMQPYLKNRNPRIRAKASMCFSKSVP 227
L LKSSQDKRFVCEAAE AL +MT +++P+ LLPK+QP LKNRNPRIRAKAS+CFS+SVP
Sbjct: 173 LLLKSSQDKRFVCEAAEKALTAMTKYVSPTLLLPKLQPCLKNRNPRIRAKASLCFSRSVP 232
Query: 228 SLGVEGIKEYGMDKLVQIAATQLSDQLPESREAARKLALELQAFYEKSQASSSGEVXXXX 287
LGVEGIKEYG+DKLVQ AA+QLSDQLPESREAAR + LELQ+ YEK+ + E
Sbjct: 233 RLGVEGIKEYGIDKLVQAAASQLSDQLPESREAARTVLLELQSVYEKAHPLINPETSSPE 292
Query: 288 XXXXXXXXXXGAESWEAFCQSKLSPLSAQAILRVTS----TTKEG-VAVGVTST 336
+WE FC+SKLS LSAQA+LRVT+ T +EG V G +S+
Sbjct: 293 EQQIPEVEPI---TWETFCKSKLSALSAQAVLRVTNVVTVTAREGLVTTGSSSS 343
>AT3G01450.1 | chr3:169903-171379 REVERSE LENGTH=327
Length = 326
Score = 301 bits (772), Expect = 2e-82, Method: Compositional matrix adjust.
Identities = 172/347 (49%), Positives = 224/347 (64%), Gaps = 37/347 (10%)
Query: 1 MSDSALKDL-NLAQSAELEKTKDSSAKSCITKPVLNGNKCNNTEENAPPVLPDAVTNGCE 59
M+ ALKDL NL S E+ D K + K N T E+ PP +
Sbjct: 1 MAAKALKDLKNLPVS---ERNIDYKTKLLV-------GKMNGTAEDKPPQNSVPFDHNHP 50
Query: 60 AGN-----------ADVEYIDSESLTDLEDAGATLSTLVARLDSKDWVMTCEALNNVRQL 108
G+ ++EYI+S+ L ++ A L +LV LDSKDWV+ C+ALN +R+L
Sbjct: 51 KGDEIEKPEAERVIVEIEYIESKDLNNVTQVDAVLKSLVTELDSKDWVLVCDALNTIRRL 110
Query: 109 AIFHKDRLQELLEPLVPLIVKSVKNPRSAVCKTALMTCADIFKAYGDLMVDSIDPLLVQL 168
+IFHK+ + +LE ++ IVKS+KNPRSAV KTA MT ADIF +Y D +D +D LL QL
Sbjct: 111 SIFHKEEMLHMLEKVILFIVKSLKNPRSAVSKTACMTSADIFSSYNDHTIDQLDLLLTQL 170
Query: 169 FLKSSQDKRFVCEAAEAALISMTSWIAPSALLPKMQPYLKNRNPRIRAKASMCFSKSVPS 228
LKSSQDKRFVCEAAE AL++MT+ ++P+ LLPK+QP+LKNRNPRIRAKAS CFS+ VP
Sbjct: 171 LLKSSQDKRFVCEAAEKALVAMTAHVSPALLLPKLQPFLKNRNPRIRAKASTCFSRCVPR 230
Query: 229 LGVEGIKEYGMDKLVQIAATQLSDQLPESREAARKLALELQAFYEKSQASSSGEVXXXXX 288
LG+EGI+EYG++KLVQ A++QLSDQLPESREAAR + LELQ Y+K+ E
Sbjct: 231 LGIEGIREYGIEKLVQAASSQLSDQLPESREAARAVLLELQTVYKKTTNVEPKEEHPEPV 290
Query: 289 XXXXXXXXXGAESWEAFCQSKLSPLSAQAILRVTS---TTKEGVAVG 332
+W+ FCQS LSPLSAQA++RVT+ +EG+ G
Sbjct: 291 ------------TWQIFCQSNLSPLSAQAVIRVTNVAGVAREGLVAG 325
>AT4G15830.1 | chr4:8995285-8996548 REVERSE LENGTH=297
Length = 296
Score = 207 bits (526), Expect = 9e-54, Method: Compositional matrix adjust.
Identities = 112/276 (40%), Positives = 174/276 (63%), Gaps = 21/276 (7%)
Query: 55 TNGCEAGNADVEYIDSESLTDLEDAGATLSTLVARLDSKDWVMTCEALNNVRQLAIFHKD 114
N + VEY+ SE+L D +++ L+ L SKDW+ C++LNN R+ AI H
Sbjct: 35 NNPVAVAESTVEYVASENLKPFSDPESSVQRLLEELASKDWIKVCDSLNNTRRFAIHHSS 94
Query: 115 RLQELLEPLVPLIVKSVKNPRSAVCKTALMTCADIFKAYGDLMVD-----SIDPLLVQLF 169
L +LE L+ ++VK++KNPRSA+CKT++MTC+DIF AYG+ +++ S+D LL+QL
Sbjct: 95 LLLPILEKLIVVMVKAMKNPRSALCKTSIMTCSDIFTAYGEKLLEGPHLKSMDDLLLQLL 154
Query: 170 LKSSQDKRFVCEAAEAALISMTSWIAPSALLPKMQPYLKNRNPRIRAKASMCFSKSVPSL 229
+K+SQDK+FVCE AE AL +M + +A LL K+Q Y+++ NPR+RAKA++ S V +
Sbjct: 155 MKASQDKKFVCEEAEKALNTMVNSVARLPLLRKLQSYVRHSNPRVRAKAAVSTSNCVSKM 214
Query: 230 GVEGIKEYGMDKLVQIAATQLSDQLPESREAARKLALEL-QAFYEKSQASSSGEVXXXXX 288
V ++E+GM L Q+AA QLSD+LPE+REAAR + L + F + G
Sbjct: 215 EVNEMEEFGMILLAQMAADQLSDKLPEAREAARSMVNSLFEKFTWNEEEDEEG------- 267
Query: 289 XXXXXXXXXGAESWEAFCQSKLSPLSAQAILRVTST 324
E+W+ FC+ ++ L+AQA++++ ++
Sbjct: 268 --------SKQEAWKKFCEKNVTGLNAQAMIKIVAS 295
>AT3G18530.1 | chr3:6373535-6375050 REVERSE LENGTH=362
Length = 361
Score = 155 bits (391), Expect = 4e-38, Method: Compositional matrix adjust.
Identities = 104/248 (41%), Positives = 150/248 (60%), Gaps = 14/248 (5%)
Query: 1 MSDSALKDL-NLAQSAELEKTKDSSAKSCITKPVLNGNKCNNTEENAPPVLPDAVTNG-- 57
M+ ALKDL NL S E+ D C K +NG + +N+ P+ + T
Sbjct: 1 MAAKALKDLKNLPVS---ERNIDCKKNPCAGK--MNGKAEDRPPQNSVPLDHNHPTGDEI 55
Query: 58 ----CEAGNADVEYIDSESLTDLEDAGATLSTLVARLDSKDWVMTCEALNNVRQLAIFHK 113
E ++EYI S+ L ++ + A L + L ++ C+ ++ + + FH
Sbjct: 56 EKPEAERVIVELEYIKSKDLNNVAEVDAVLKVSIV-LSWYYTMLYCDFSFSLDRSSSFHF 114
Query: 114 DRLQEL-LEPLVPLIVKSVKNPRSAVCKTALMTCADIFKAYGDLMVDSIDPLLVQLFLKS 172
+ + ++ +VKS+KNPRSAV KTA MT DIF +Y D + D +D LL QL LKS
Sbjct: 115 PQGRNAAFAKVILFVVKSLKNPRSAVSKTACMTSEDIFSSYNDHIFDQLDRLLTQLLLKS 174
Query: 173 SQDKRFVCEAAEAALISMTSWIAPSALLPKMQPYLKNRNPRIRAKASMCFSKSVPSLGVE 232
SQDKRFVCEAAE AL++MT+ ++P+ LLPK++P LKN++PRIRAKAS CFS VP LG+E
Sbjct: 175 SQDKRFVCEAAERALVAMTTHVSPALLLPKLRPCLKNKSPRIRAKASACFSGCVPRLGIE 234
Query: 233 GIKEYGMD 240
G++EYG++
Sbjct: 235 GMREYGIE 242
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.127 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,622,057
Number of extensions: 238824
Number of successful extensions: 614
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 609
Number of HSP's successfully gapped: 4
Length of query: 346
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 247
Effective length of database: 8,392,385
Effective search space: 2072919095
Effective search space used: 2072919095
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 112 (47.8 bits)