BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0532900 Os08g0532900|AK073679
         (212 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G07680.1  | chr3:2455627-2456652 FORWARD LENGTH=209            297   3e-81
AT3G22845.1  | chr3:8087373-8088550 FORWARD LENGTH=215            121   2e-28

>AT3G07680.1 | chr3:2455627-2456652 FORWARD LENGTH=209
          Length = 208

 Score =  297 bits (760), Expect = 3e-81,   Method: Compositional matrix adjust.
 Identities = 136/192 (70%), Positives = 159/192 (82%)

Query: 21  RPAAAIRFVIDREECFSHNVEYEGDTVHVSFVVIKADTPWHYSEDGVDLVVKDPNGAQVR 80
           +    IRFVIDREECFSH  EYEGDT+HVSFVVIK+D+ WH++EDGVDLV+  P G Q+ 
Sbjct: 17  QATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPTGEQIH 76

Query: 81  DSRDKTSDKFEFIVQKRGVHRFCFTNKSPYHETVDFDVHVGHFSYFDQHAKDEHFAPLFE 140
           D R++ S K +F+VQK+GV+RFCFTNKSPYHET+DFDV +GHF+Y+DQHAKDEHF PL E
Sbjct: 77  DFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHFTPLME 136

Query: 141 QIAKLDEALYNIQFEQHWLEAQTDRQAILNENMSRRAVHKXXXXXXXXXXXXVIQVYLLR 200
           QI+KL+EALYNIQFEQHWLEAQTDRQAI+NENMS+RAVHK             +QVYLLR
Sbjct: 137 QISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLR 196

Query: 201 RLFERKLGTSRV 212
           RLFERKLG SRV
Sbjct: 197 RLFERKLGMSRV 208



>AT3G22845.1 | chr3:8087373-8088550 FORWARD LENGTH=215
          Length = 214

 Score =  121 bits (304), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 67/189 (35%), Positives = 99/189 (52%)

Query: 24  AAIRFVIDREECFSHNVEYEGDTVHVSFVVIKADTPWHYSEDGVDLVVKDPNGAQVRDSR 83
           +++   ++ EEC    V YEGDTV  +FVV+  D  W     G+D  V  P G  V+  +
Sbjct: 26  SSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLDFTVTSPAGNIVQTLK 85

Query: 84  DKTSDKFEFIVQKRGVHRFCFTNKSPYHETVDFDVHVGHFSYFDQHAKDEHFAPLFEQIA 143
             + DKFEF   K G+++FCF N     ETV F +HVGH       AKDEH  P+  +IA
Sbjct: 86  GTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIA 145

Query: 144 KLDEALYNIQFEQHWLEAQTDRQAILNENMSRRAVHKXXXXXXXXXXXXVIQVYLLRRLF 203
           +L EAL ++  EQ +L+A+  R    NE+  +R +               +QV  +R+LF
Sbjct: 146 ELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYIFLAAASGLQVLYIRKLF 205

Query: 204 ERKLGTSRV 212
            + +  +RV
Sbjct: 206 SKSVAYNRV 214



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.137    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,980,482
Number of extensions: 159546
Number of successful extensions: 445
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 446
Number of HSP's successfully gapped: 2
Length of query: 212
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 118
Effective length of database: 8,529,465
Effective search space: 1006476870
Effective search space used: 1006476870
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 109 (46.6 bits)