BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0526500 Os08g0526500|AK073431
         (237 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G48680.1  | chr5:19744725-19746053 REVERSE LENGTH=207           65   4e-11
AT3G07170.1  | chr3:2280428-2282097 FORWARD LENGTH=204             63   2e-10

>AT5G48680.1 | chr5:19744725-19746053 REVERSE LENGTH=207
          Length = 206

 Score = 64.7 bits (156), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 53/176 (30%), Positives = 77/176 (43%), Gaps = 25/176 (14%)

Query: 46  KRFRQADGTWRRELYMDXXX---XXXXXXXXXRNIQGNQKPHLQQNTQVMRKSSVPDLRE 102
           KR RQ D  W  +L+ D                 ++  +K H  Q    +   SV DLR+
Sbjct: 39  KRGRQDDDKWEHDLFEDDDEPRLSKRRVDPKDLRLKLQKKRHGSQIGGRVFSVSVADLRD 98

Query: 103 KLSGVQRPQLNSTVQ--IPKSVPEISTSAKPVQKREPVQKREPPVNAALPATKKVNEPSA 160
           KLS    PQ  ++ +  +  ++ ++S   KP  +  P        N A     + N+ S 
Sbjct: 99  KLSRTVNPQTKNSKREAVRPAIKKVSVGTKPETRAAP--------NRATKKDPQQNDASV 150

Query: 161 PKQCQEKTXXXXXXXXXXXXVEKYSINFQAEEVDMKALLHMNEEDMKSLGIPMGPR 216
               +               +EKYS  FQ EEVDM AL HM ++D+K+L IPMGPR
Sbjct: 151 DSFLE------------SLGLEKYSTAFQVEEVDMDALRHMTDDDLKALLIPMGPR 194



>AT3G07170.1 | chr3:2280428-2282097 FORWARD LENGTH=204
          Length = 203

 Score = 62.8 bits (151), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 64/220 (29%), Positives = 95/220 (43%), Gaps = 32/220 (14%)

Query: 1   MYSDSV---SGRKRSVMDRLXXXXXXXXXXXXXXXXXXXXXRPRPDSVKRFRQADGTWRR 57
           MY+D +   SG ++ V DR+                     R R  + KR RQ D  W  
Sbjct: 1   MYADKLEAESGSRKVVKDRINGGSGDIST------------RVRQVTGKRQRQ-DDKWEH 47

Query: 58  ELYMDXXXXXXXXXXXXRNIQGN-QKPHLQQNTQVMRKSSVPDLREKLSGVQRPQLNSTV 116
           +L+              R+++   QK H    +     S V DLR++LSG    Q  ++ 
Sbjct: 48  DLFSSDKPQLSNRRVDPRDLRLKLQKRHHGSQSGREAGSGVRDLRDQLSGTMNQQPKNS- 106

Query: 117 QIPKSVPEISTSAKPVQKREPVQKREPPVNAALPATKKVNEPSAPKQCQEKTXXXXXXXX 176
             PKS  E   +A+P  K    +            T+K +  +  K+ Q+          
Sbjct: 107 DPPKSKAE---AARPSMKSVATE----------TETRKTSSQATRKKSQQ-ADSSVDSFL 152

Query: 177 XXXXVEKYSINFQAEEVDMKALLHMNEEDMKSLGIPMGPR 216
               +EKYS  FQ EEVDM AL+HM ++D+K++ IPMGPR
Sbjct: 153 ESLGLEKYSTAFQVEEVDMDALMHMTDDDLKAMLIPMGPR 192



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.311    0.126    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,654,478
Number of extensions: 119389
Number of successful extensions: 390
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 390
Number of HSP's successfully gapped: 2
Length of query: 237
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 141
Effective length of database: 8,474,633
Effective search space: 1194923253
Effective search space used: 1194923253
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 110 (47.0 bits)