BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0518900 Os08g0518900|AK073267
         (315 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G24090.1  | chr5:8143805-8145153 REVERSE LENGTH=303            155   3e-38

>AT5G24090.1 | chr5:8143805-8145153 REVERSE LENGTH=303
          Length = 302

 Score =  155 bits (391), Expect = 3e-38,   Method: Compositional matrix adjust.
 Identities = 94/282 (33%), Positives = 149/282 (52%), Gaps = 17/282 (6%)

Query: 39  RRNVVVFWGGNKNEGSLRSVCDSGLYNIVIISFYSLFGHGRYWD-DLSGH------DLRH 91
           R  + ++WG N NEG+L + C +G Y  V ++F   FG+G+  + +L+GH         H
Sbjct: 29  RGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTH 88

Query: 92  IGADITHCHFKAVYVLLSIGGGDGKDYSLPSSKSAADVADNLYNSFLGGSRPGVYHPFGD 151
            G+ +  C  + + V+LS+GGG G +YS+ S + A  +AD L+N+FLGG       P GD
Sbjct: 89  FGSQVKDCQSRGIKVMLSLGGGIG-NYSIGSREDAKVIADYLWNNFLGGKSSS--RPLGD 145

Query: 152 DVTVVGIDFFIDRGQPDHYYEIAERINYDTRHWRDPIGFKLTATVSCAYDDSDPRMKKAL 211
            V + GIDF I+ G P H+ ++A  ++  +   R      LT    C + D    M  AL
Sbjct: 146 AV-LDGIDFNIELGSPQHWDDLARTLSKFSHRGRK---IYLTGAPQCPFPDR--LMGSAL 199

Query: 212 ETYLFRRIHVRFYDDPRCSYNHAGLAGVMAQWNRWSASYPYNGKIYLGLAAANLTGKNDM 271
            T  F  + ++FY++P CSY+      +   WN+W+ S     K +LGL AA     +  
Sbjct: 200 NTKRFDYVWIQFYNNPPCSYSSGNTQNLFDSWNKWTTSIAAQ-KFFLGLPAAPEAAGSGY 258

Query: 272 VAVGELYRKLLPAVQKTDTYGGVMLWNSYYDSITHYGRYVSA 313
           +    L  ++LP ++K+  YGGVMLW+ ++D    Y   + A
Sbjct: 259 IPPDVLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSILA 300



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.139    0.449 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,345,852
Number of extensions: 330841
Number of successful extensions: 568
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 563
Number of HSP's successfully gapped: 1
Length of query: 315
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 217
Effective length of database: 8,419,801
Effective search space: 1827096817
Effective search space used: 1827096817
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)