BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0518800 Os08g0518800|AK064180
         (181 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G24090.1  | chr5:8143805-8145153 REVERSE LENGTH=303             99   9e-22

>AT5G24090.1 | chr5:8143805-8145153 REVERSE LENGTH=303
          Length = 302

 Score = 99.4 bits (246), Expect = 9e-22,   Method: Compositional matrix adjust.
 Identities = 62/188 (32%), Positives = 96/188 (51%), Gaps = 17/188 (9%)

Query: 1   SLPTSQSAADVADNLWNAFLAGRRAGVSRPFGHEAAVDGVDFFIDQGGADHYDELARRLH 60
           S+ + + A  +AD LWN FL G+ +  SRP G +A +DG+DF I+ G   H+D+LAR L 
Sbjct: 115 SIGSREDAKVIADYLWNNFLGGKSS--SRPLG-DAVLDGIDFNIELGSPQHWDDLARTLS 171

Query: 61  GY---GAGVIWTATTRCSYPDHRLEKALATKVFDRIHVRMYGAGEIERRCVISSRY---- 113
            +   G  +  T   +C +PD  +  AL TK FD + ++ Y        C  SS      
Sbjct: 172 KFSHRGRKIYLTGAPQCPFPDRLMGSALNTKRFDYVWIQFYN----NPPCSYSSGNTQNL 227

Query: 114 --SWEKWAAAYPGSKVYIGLVASPEQDEAWVFQKD-LYYEYLQFVTKLPNYGGLAVYDRY 170
             SW KW  +    K ++GL A+PE   +     D L  + L  + K   YGG+ ++ ++
Sbjct: 228 FDSWNKWTTSIAAQKFFLGLPAAPEAAGSGYIPPDVLTSQILPTLKKSRKYGGVMLWSKF 287

Query: 171 YDKKANYT 178
           +D K  Y+
Sbjct: 288 WDDKNGYS 295



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.137    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,457,254
Number of extensions: 186454
Number of successful extensions: 290
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 286
Number of HSP's successfully gapped: 1
Length of query: 181
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 89
Effective length of database: 8,584,297
Effective search space: 764002433
Effective search space used: 764002433
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 108 (46.2 bits)