BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0517300 Os08g0517300|AK069175
         (647 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G27100.1  | chr2:11572587-11576357 FORWARD LENGTH=721          565   e-161

>AT2G27100.1 | chr2:11572587-11576357 FORWARD LENGTH=721
          Length = 720

 Score =  565 bits (1456), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 312/574 (54%), Positives = 386/574 (67%), Gaps = 48/574 (8%)

Query: 101 FNRAPDWTDSGRGGWNDGP--ANSRREGLMSYKQFMQELEDDVSPDEAQHRYEEYKSEYI 158
             R  DW + GRGG+ D     N +R+GLMSYKQF+QELEDD+ P EA+ RY+EYKSEYI
Sbjct: 168 MGRYQDW-EGGRGGYGDASNSGNPQRDGLMSYKQFIQELEDDILPSEAERRYQEYKSEYI 226

Query: 159 TTQKKAYFDLHKNDDWLKNKYHPTNLEIVMERRNELARTAANQFLQDLQNGSLDIGPGLT 218
           TTQK+A+F+ HK +DWLKNKYHPTNL  V+ERRN+LA+  A  FL DLQ+G+LD+GP +T
Sbjct: 227 TTQKRAFFNTHKEEDWLKNKYHPTNLLSVIERRNDLAQKVAKDFLLDLQSGTLDLGPAVT 286

Query: 219 SSATNKSG-----NSVDDTDANGKK---GKLGKSQDDLYYAAPKAHPVSSEPRRIRIDVE 270
             A NKSG     NS D+    GK+   G  G  +++L  AAPKA   +S+P+RI  DVE
Sbjct: 287 --ALNKSGRTSEPNSEDEAAGVGKRKRHGMGGAKENELLSAAPKAPSFTSDPKRILTDVE 344

Query: 271 LAQALICKLDSEKGIENNVLSGSDHDRAEKDKSH-GSMGPIVIIRGSSAVKGLEGAELLD 329
             QAL+ KLDSEK IE NVL GS+ +++ ++K H GS GP+VIIRG ++VKGLEG ELLD
Sbjct: 345 QTQALVRKLDSEKKIEENVLQGSETEKSGREKLHSGSTGPVVIIRGLTSVKGLEGVELLD 404

Query: 330 TLVTYLWRIHGVDYYGMSETNEPKGLRHVKADSRTFNGASSNAAEWEKKLDSFWQDRIQG 389
           TLVTYLWR+HG+DYYG  ETNE KGLRHV+A+ +    + +   E E K DS WQ+R++G
Sbjct: 405 TLVTYLWRVHGLDYYGKVETNEAKGLRHVRAEGKV---SDAKGDENESKFDSHWQERLKG 461

Query: 390 QDPMEILXXXXXXXXXXSEVLDPHVRKIRDEKYGWKYGCGAKGCTKLFHAPEFVQKHLKL 449
           QDP+E++          +E LDPHVRKIRDEKYGWKYGCGAKGCTKLFHA EFV KHLKL
Sbjct: 462 QDPLEVMAAKEKIDAAATEALDPHVRKIRDEKYGWKYGCGAKGCTKLFHAAEFVYKHLKL 521

Query: 450 KHADLVRELTSKVRENIYFDNYMSDPKAPGGMPIMQQPAPREK-VRQRPPIESRLRDE-- 506
           KH +LV ELT+KVRE +YF NYM+DP APGG P  QQ  PR++ +R++P +E+RLRD+  
Sbjct: 522 KHTELVTELTTKVREELYFQNYMNDPNAPGGQPATQQSGPRDRPIRRKPSMENRLRDDRG 581

Query: 507 ---------RGNRRFDRN-DSPTHDGSSDNPDD-AYDSYGDP--VMHGAFPPDIPAPPVL 553
                     GN R DR+ D    D    NP +  YD++G    V    F  DI  PP+L
Sbjct: 582 GRRERDGRANGNDRNDRSEDQQRGDNDGGNPGEVGYDAFGGQGGVHVPPFLSDINPPPML 641

Query: 554 MPVPGAGPLGPFIPAPPEVAMRMMRDQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 613
           MPVPGAGPLGPF+PAPPEVAM+M RD                                  
Sbjct: 642 MPVPGAGPLGPFVPAPPEVAMQMFRD---------------PSGPNPPFEGSGRGGPAPF 686

Query: 614 XXXXXHMHDPRKIRSYQDLDAPEDEVTVMDYRSL 647
                   DPR++RSYQDLDAPE+EVTV+DYRSL
Sbjct: 687 LLSPAFRQDPRRLRSYQDLDAPEEEVTVIDYRSL 720



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.134    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 13,021,674
Number of extensions: 568726
Number of successful extensions: 1748
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1750
Number of HSP's successfully gapped: 2
Length of query: 647
Length of database: 11,106,569
Length adjustment: 105
Effective length of query: 542
Effective length of database: 8,227,889
Effective search space: 4459515838
Effective search space used: 4459515838
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 115 (48.9 bits)