BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0516000 Os08g0516000|AK072130
         (790 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G25070.1  | chr5:8641261-8643471 REVERSE LENGTH=737            228   1e-59

>AT5G25070.1 | chr5:8641261-8643471 REVERSE LENGTH=737
          Length = 736

 Score =  228 bits (580), Expect = 1e-59,   Method: Compositional matrix adjust.
 Identities = 156/462 (33%), Positives = 255/462 (55%)

Query: 244 RLALLRSQISGKLESIQQRATAVAAKRRQLAGRRRKVAEDVGSAASRHKXXXXXXXXXXX 303
           RL L+++QI  KL   +  A +V + R+    ++R+ +E++  A++ H+           
Sbjct: 188 RLELVKAQIEAKLNRSRDLAASVTSARKNAIRKKRQASENLRLASTTHEELEKQLEEAIE 247

Query: 304 XXXXXXXXKISDSLAALEKDKDRLLTALRDAELNYDSVDLELQDVLDSRIXXXXXXXXXX 363
                   +IS+SLAA E+D+  LL  LR AE + D+++ ++++VL S+I          
Sbjct: 248 TEDFDAAERISESLAAKERDRLALLALLRQAESDCDAIESKMEEVLLSQIAAEEESACLL 307

Query: 364 XHFAKDATDHTDSASKEAEEISLKEIEGWRXXXXXXXXXXXXXXXXXXXXXAARSGLEDS 423
             F  DA +   S  ++AE     E+E W                        R  L   
Sbjct: 308 RSFGTDAENDAGSILEKAEAFYSDEMEKWHSCSEDVEVRKVELDIESVVVDNVRLSLNGI 367

Query: 424 IEHLVEDDKREKDMLSKKGDXXXXXXXXXXXXVRLKEAEIAENNAQIQEVQERITAVVSR 483
           +E  VE D +EK++L KK +            V+ KE EI EN++QI+ V+ERI  VV+ 
Sbjct: 368 LEGSVEQDMKEKEILQKKKEHLANELEELLALVKAKEKEIDENDSQIEAVEERINNVVTG 427

Query: 484 FYGSQTDIDLKLKTLQEAQTKMDSEAQALALKKNEIDSFISLSEQKDSKLREIINDCSSE 543
           F   QT +D  L  +Q   T++D E + L+ KK ++D F++  +++ +KLR++    + E
Sbjct: 428 FKELQTSMDKMLNDVQAGLTEVDKETEDLSRKKKDVDEFMTSEKERGAKLRDLARVSADE 487

Query: 544 AKACQQSVDIRRKLASSILKSREDRIGLLKMEEEILQDIQVLRQQTSDARISLQEISSRK 603
           A   ++ + +R+ L S + K+RE+R  L+ +EE++ +++Q L+++ S  R  L+E SS+K
Sbjct: 488 ACEYEEVIKLRKGLMSYVSKTREERAKLVNIEEKLSEEVQKLQEEVSSTRELLKERSSKK 547

Query: 604 AVIQQDIATFKQKLSFIDKRGPELEAEKRVAAAARNFKEAGRIAAEAKALYSXXXXXXXX 663
           ++IQQ+I +F  K+ FI+KR PELEAEK+VAA+ RNFKEAGRIAAEAK+L          
Sbjct: 548 SIIQQNITSFMDKIMFIEKRMPELEAEKKVAASTRNFKEAGRIAAEAKSLNLEKDKTQME 607

Query: 664 XXXXXTDLEVIEKDITATTDKIQECEGLIILKEKESAMTSYK 705
                 +LE  E +I  T  ++QE E LI+ KEKE A++ ++
Sbjct: 608 TGKANAELEKAEHEIEETIKRLQEIEKLILSKEKELAISRFQ 649



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.128    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,808,789
Number of extensions: 282389
Number of successful extensions: 1057
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1068
Number of HSP's successfully gapped: 2
Length of query: 790
Length of database: 11,106,569
Length adjustment: 106
Effective length of query: 684
Effective length of database: 8,200,473
Effective search space: 5609123532
Effective search space used: 5609123532
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 116 (49.3 bits)