BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0485400 Os08g0485400|AK068155
         (280 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G64250.2  | chr5:25697623-25698945 REVERSE LENGTH=334          335   2e-92

>AT5G64250.2 | chr5:25697623-25698945 REVERSE LENGTH=334
          Length = 333

 Score =  335 bits (858), Expect = 2e-92,   Method: Compositional matrix adjust.
 Identities = 164/267 (61%), Positives = 199/267 (74%)

Query: 4   KGILGFDHGVVQAPLGPDISGPELAAAVANAGGIGLLRLPDWPAPDRVRDLIRRTRSLTE 63
           KGILGF++G+VQAPLGPDISGPEL AAVANAGGIGLLR PDW  PD +R+LIR+T++LTE
Sbjct: 2   KGILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTE 61

Query: 64  RPFGAAIVLAFPHEENLRVVLEEKLAVLQVYWGEFPRERVDEAHLAGVKVLHQVGSFEEA 123
           +PFG  +VLAFPH+ N++ +LEEK+AVLQ+YWG+  +E VD+AH AGVKV+ QVG+ EEA
Sbjct: 62  KPFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEA 121

Query: 124 AKAKEAGVDGIIVQGCEAGGHVIGQEGXXXXXXXXXXXXSDTDISVIAAGGIVDXXXXXX 183
            KA   GVD IIVQG EAGGHVIG++G             + DI VIAAGGIVD      
Sbjct: 122 RKAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVA 181

Query: 184 XXXXXXXXXXXXTRFLTTEESFAHPLYKKRLIEINCTGYTNVFGRARWPGAPQRVLKTTF 243
                       TRF+ T ES+AHP+YK++LIE   T YT+VFGRARWPGAPQRVL+T F
Sbjct: 182 ALSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDVFGRARWPGAPQRVLETPF 241

Query: 244 YDQWKNLPEQETEENQPIIGHTIIHGV 270
           +D W++LP  E E NQPIIG + IHGV
Sbjct: 242 FDDWRSLPAHENEVNQPIIGRSTIHGV 268



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,576,263
Number of extensions: 209975
Number of successful extensions: 451
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 450
Number of HSP's successfully gapped: 1
Length of query: 280
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 183
Effective length of database: 8,447,217
Effective search space: 1545840711
Effective search space used: 1545840711
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)