BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0461800 Os08g0461800|AK067364
         (243 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G78760.1  | chr1:29615174-29616738 REVERSE LENGTH=453           49   3e-06

>AT1G78760.1 | chr1:29615174-29616738 REVERSE LENGTH=453
          Length = 452

 Score = 48.5 bits (114), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 59/227 (25%), Positives = 106/227 (46%), Gaps = 24/227 (10%)

Query: 28  IQAPNLERLTITSDIDYGWQIQDLPSIQDANINI------EDYSIDRDFVKLLTS-LAQV 80
           I AP LE L I+      +++++  S+ +A INI        +  DR   K++   LA++
Sbjct: 235 IDAPKLEYLNISDHSTAKFKMKNSGSLVEAKINIIFNMEELPHPNDRPKRKMIQDFLAEI 294

Query: 81  GEL-ELFIPSADGNVLEGISCSFQKLRSL-TLHTNFYKAS-SILSTFGLLTRAPNLLHLE 137
             + +LFI S    V+  + C     R+L +LH +F   +  +LSTF  L   PN+  L 
Sbjct: 295 SSVKKLFISSHTLEVIHDLGCELPLFRNLSSLHIDFEDHTLKMLSTF--LQSCPNVKSLV 352

Query: 138 IEITDHENQSDEVDIDFLNALWTNSLFANLDFVSI-KSATCWSNEMRFIEFVLSKARLLG 196
           +E  D    S + D D + ++     F  L++V I +  T  +  M+ + ++L  + +L 
Sbjct: 353 VEFKD----SSKEDGDRVLSI-PRCFFTTLEYVKIERPITGEARGMKVVSYILENSPILK 407

Query: 197 EFYIYHDDTGSYSKPREEAII--ELAKYKRASPKAKVFFRDMEDTWL 241
           +  +  +     S+ + E++I  EL    R S   KV   +    W+
Sbjct: 408 KLNLCLNS----SREKSESVILKELLTIPRLSTSCKVVVFEPRSCWV 450



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,293,803
Number of extensions: 215731
Number of successful extensions: 654
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 656
Number of HSP's successfully gapped: 1
Length of query: 243
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 147
Effective length of database: 8,474,633
Effective search space: 1245771051
Effective search space used: 1245771051
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 110 (47.0 bits)