BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0459300 Os08g0459300|AK060409
         (226 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G02180.1  | chr1:413619-414505 REVERSE LENGTH=227              134   3e-32

>AT1G02180.1 | chr1:413619-414505 REVERSE LENGTH=227
          Length = 226

 Score =  134 bits (338), Expect = 3e-32,   Method: Compositional matrix adjust.
 Identities = 77/195 (39%), Positives = 110/195 (56%), Gaps = 6/195 (3%)

Query: 33  NPADQLVALINSNRTASKASTLDDNQGLGCIALQYIKAYEGQCNQVGE-SKKPPETSFAE 91
           N A ++V+++N NRTA K   L+++ GLGC+ALQY++  EG CN     S + PE  F +
Sbjct: 29  NSAHEMVSILNQNRTARKLGKLNESPGLGCMALQYVELCEGNCNVNNTLSCEHPEDDFTQ 88

Query: 92  TFAPNCGVQAATLTKITGRLLACQSNYATPDQAF-NFLVNDAKSIQVLHSKNHTEXXXXX 150
            FAPNCGV+  T   ITG +L C S YA P+ AF + L  D+ ++ VL +++HTE     
Sbjct: 89  VFAPNCGVELPTFGTITGHILGCSSKYAAPEVAFSDILFRDSSALSVLRNRSHTEVGVGM 148

Query: 151 XXXXXXXPYFWCVLFSSGKPTTSFKV-DGGVPKSVRPGCFSGNNDDCMGANAAVSIGAGT 209
                   +FWC+LFS G   +SF + D G     R GC+SG+   C  ++ A  I    
Sbjct: 149 ARLHKGT-FFWCLLFSDGVKNSSFVLEDNGRGIKQRTGCYSGSAFPC--SSNAHMICMRL 205

Query: 210 WRLVAALLFSAACVF 224
                 LLFS+ C+F
Sbjct: 206 LNSFLGLLFSSFCLF 220



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.130    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,226,208
Number of extensions: 154052
Number of successful extensions: 254
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 252
Number of HSP's successfully gapped: 1
Length of query: 226
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 131
Effective length of database: 8,502,049
Effective search space: 1113768419
Effective search space used: 1113768419
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)