BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0458200 Os08g0458200|AK061399
(232 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G43850.1 | chr3:15702085-15704152 REVERSE LENGTH=271 57 7e-09
AT5G21940.1 | chr5:7249446-7250353 REVERSE LENGTH=265 53 2e-07
>AT3G43850.1 | chr3:15702085-15704152 REVERSE LENGTH=271
Length = 270
Score = 57.4 bits (137), Expect = 7e-09, Method: Compositional matrix adjust.
Identities = 29/52 (55%), Positives = 38/52 (73%), Gaps = 2/52 (3%)
Query: 66 PIRRSISKFYNGKSKSFACLKEAVSSCGSAKDIAKAESAYSRKRKNLLAYSI 117
PI+R+ISKFY GKSKSF L E +S KD+ K E+ YSR+R+NLL++ I
Sbjct: 63 PIKRAISKFYKGKSKSFMSLSE--TSSLPVKDLTKPENLYSRRRRNLLSHRI 112
>AT5G21940.1 | chr5:7249446-7250353 REVERSE LENGTH=265
Length = 264
Score = 52.8 bits (125), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 38/89 (42%), Positives = 54/89 (60%), Gaps = 9/89 (10%)
Query: 66 PIRRSISKFYNGKSKSFACL----KEAVSSCGSAKDIAKAESAYSRKRKNLLAYSIMYET 121
P+R+ ISK+Y+GKSKSF L A++S S KD+AK E+ YSR+R+NLL + I +E
Sbjct: 86 PVRKGISKYYSGKSKSFTNLTAEAASALTSSSSMKDLAKPENPYSRRRRNLLCHQI-WEN 144
Query: 122 SQETAAEVYETGPPKRLASLSRNSLVTLA 150
++ T G K+ S S +TLA
Sbjct: 145 NKTTP----RGGISKKHVMSSSRSALTLA 169
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.310 0.124 0.344
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,804,040
Number of extensions: 109363
Number of successful extensions: 192
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 194
Number of HSP's successfully gapped: 2
Length of query: 232
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 137
Effective length of database: 8,502,049
Effective search space: 1164780713
Effective search space used: 1164780713
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 110 (47.0 bits)