BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0414300 Os08g0414300|AK072217
(172 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G38060.2 | chr4:17873709-17874379 REVERSE LENGTH=137 56 1e-08
AT5G65480.1 | chr5:26176827-26177434 FORWARD LENGTH=154 46 8e-06
>AT4G38060.2 | chr4:17873709-17874379 REVERSE LENGTH=137
Length = 136
Score = 55.8 bits (133), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 32/71 (45%), Positives = 40/71 (56%), Gaps = 7/71 (9%)
Query: 107 WSIGWLEPQGPELHSDGD-SDGSFAVLVPCYR---HGRCRVEEP---GRGRLADGNVSGG 159
WS+GW EP GP+ SD + +DG F VLVPCYR G L +G G
Sbjct: 63 WSVGWTEPHGPDFQSDDEGNDGGFLVLVPCYRAVVEGSSNNNNQLLSAVKNLPNGLPPDG 122
Query: 160 KNFVERWLSSL 170
KN++E+WLSSL
Sbjct: 123 KNYMEQWLSSL 133
>AT5G65480.1 | chr5:26176827-26177434 FORWARD LENGTH=154
Length = 153
Score = 46.2 bits (108), Expect = 8e-06, Method: Compositional matrix adjust.
Identities = 28/68 (41%), Positives = 40/68 (58%), Gaps = 9/68 (13%)
Query: 107 WSIGWLEPQGPELHSDGDSDGSFAVLVPCYRHGRCRVEEPGRGRLADGNVS----GGKNF 162
WSIGW+EP GP ++ D G F VLVP Y+ +V + ++ +G S GKN
Sbjct: 88 WSIGWVEPHGPGFKAEDDDCGGFVVLVPLYK----KVMDGSGNQIPNGFFSPAAPDGKN- 142
Query: 163 VERWLSSL 170
+E+WLSS+
Sbjct: 143 MEQWLSSM 150
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.319 0.140 0.474
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,888,670
Number of extensions: 89771
Number of successful extensions: 142
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 140
Number of HSP's successfully gapped: 2
Length of query: 172
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 80
Effective length of database: 8,584,297
Effective search space: 686743760
Effective search space used: 686743760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 108 (46.2 bits)