BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0386900 Os08g0386900|AK058827
         (356 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G46180.1  | chr2:18967482-18971260 REVERSE LENGTH=726          270   7e-73
AT3G61570.1  | chr3:22779637-22783642 REVERSE LENGTH=713          257   5e-69

>AT2G46180.1 | chr2:18967482-18971260 REVERSE LENGTH=726
          Length = 725

 Score =  270 bits (691), Expect = 7e-73,   Method: Compositional matrix adjust.
 Identities = 159/345 (46%), Positives = 204/345 (59%), Gaps = 26/345 (7%)

Query: 1   MDEDSKLIEELRAICDQQRAHIVQLERALKFEMAKQXXXXXXXXXXHQRSNEQITDLKYK 60
           MDEDS+LI+ELR   + QR+ I+ LE+AL+  MA Q           ++S   I DL  K
Sbjct: 392 MDEDSRLIDELRQTNEYQRSQILGLEKALRQTMANQEEIKSSSDLEIRKSKGIIEDLNQK 451

Query: 61  LANCMNALESKNLELLNLQTALGQYYAESEAKERLGGDLAMAREELAKLSESLKVANQAI 120
           LANC+  ++SKN+ELLNLQTALGQYYAE EAKE    +LA+A+E+  KLS  LK  ++ +
Sbjct: 452 LANCLRTIDSKNVELLNLQTALGQYYAEIEAKEHFERELAVAKEDAMKLSARLKDVDEQL 511

Query: 121 EISRREKEEVAARLSQVEGMLADGKRSMQKLENDNSRLRRALEQSMTTLNRMSLDSDNSV 180
           E S++EKEE+ +++   E + A+ K  + K+E+DN+++RR LEQSMT LNRMS+DSD  V
Sbjct: 512 ESSKKEKEEITSKVLHAENIAAEWKNRVSKVEDDNAKVRRVLEQSMTRLNRMSMDSDFLV 571

Query: 181 DRRIVIKLLVTYFQRNHSKEVLDLMVRMLGFSEEDKQRIGFAQSNAXXXXXXXXX----X 236
           DRRIVIKLLVTYFQRNHS+EVLDLMVRMLGFSEE+KQRIG AQ  A              
Sbjct: 572 DRRIVIKLLVTYFQRNHSREVLDLMVRMLGFSEEEKQRIGLAQQGAAGKGVVRGVLGFPG 631

Query: 237 XXXXXXXXXXXXNSAGKPTQASQDNQSFADLWVDFXXXXXXXXXXXXXXXXXRLSQEEXX 296
                       +    P  AS DNQSFAD+WV+F                    QE   
Sbjct: 632 RLVGGILGGGGGSPDSHPNMAS-DNQSFADMWVEFLLKDAEERERREAEDAANKEQE--- 687

Query: 297 XXXXXXXXXXXPSGHISNQAPGPSTSHHMFGRQDTEFATVPLTSS 341
                             +A   ST    + + D+EF+TVPLTSS
Sbjct: 688 ------------------KATVSSTQRPKYEQSDSEFSTVPLTSS 714



>AT3G61570.1 | chr3:22779637-22783642 REVERSE LENGTH=713
          Length = 712

 Score =  257 bits (657), Expect = 5e-69,   Method: Compositional matrix adjust.
 Identities = 142/271 (52%), Positives = 181/271 (66%)

Query: 1   MDEDSKLIEELRAICDQQRAHIVQLERALKFEMAKQXXXXXXXXXXHQRSNEQITDLKYK 60
           MDEDS+LIEELR   + QR+ I  LE++LK  ++ Q           ++  + + DL  K
Sbjct: 387 MDEDSRLIEELRQTNEYQRSQISHLEKSLKQAISNQEDNRLSNDNQIRKLKDTVDDLNQK 446

Query: 61  LANCMNALESKNLELLNLQTALGQYYAESEAKERLGGDLAMAREELAKLSESLKVANQAI 120
           L NC+  +ESKN+ELLNLQTALGQYYAE EAKE    +LAMA++EL KLS  LK +++ +
Sbjct: 447 LTNCLRTIESKNVELLNLQTALGQYYAEIEAKEHFERELAMAKDELMKLSARLKDSDERL 506

Query: 121 EISRREKEEVAARLSQVEGMLADGKRSMQKLENDNSRLRRALEQSMTTLNRMSLDSDNSV 180
           E S +EKE+V ++L   E + A+ K  + K+E DN+++RR LEQSMT LNRMS++SD  V
Sbjct: 507 ESSNKEKEDVTSKLLHAEKVAAEWKNRVTKVEEDNAKVRRVLEQSMTRLNRMSMESDYLV 566

Query: 181 DRRIVIKLLVTYFQRNHSKEVLDLMVRMLGFSEEDKQRIGFAQSNAXXXXXXXXXXXXXX 240
           DRRIVIKLLVTYFQ+NH+KEVLDLMVRMLGFSEEDK+RIG A+                 
Sbjct: 567 DRRIVIKLLVTYFQKNHNKEVLDLMVRMLGFSEEDKERIGAAKQGGGKGVVRGVLGFPGR 626

Query: 241 XXXXXXXXNSAGKPTQASQDNQSFADLWVDF 271
                    SA     A+ DNQSFADLWVDF
Sbjct: 627 FVGGILGGKSAELHANAASDNQSFADLWVDF 657



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.126    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,367,163
Number of extensions: 165528
Number of successful extensions: 836
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 838
Number of HSP's successfully gapped: 2
Length of query: 356
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 256
Effective length of database: 8,364,969
Effective search space: 2141432064
Effective search space used: 2141432064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 112 (47.8 bits)