BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0327400 Os08g0327400|AK070992
         (375 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G05990.1  | chr2:2322876-2324867 FORWARD LENGTH=391            499   e-142
AT1G07440.1  | chr1:2286436-2287665 REVERSE LENGTH=267             49   5e-06

>AT2G05990.1 | chr2:2322876-2324867 FORWARD LENGTH=391
          Length = 390

 Score =  499 bits (1285), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 258/383 (67%), Positives = 289/383 (75%), Gaps = 10/383 (2%)

Query: 1   MGASAATGMQMVAARPCISASQGMLTSRAAV--SRIGRA----LSTTTGFATCPRICYSS 54
           M A+AA+ +Q+   RP +S+   +L +   +  +  G A    LS T   +    +   S
Sbjct: 1   MAATAASSLQIATRRPSMSSPSKILKAGTYIVGANPGNASWDKLSCTRQLSNLGCLRNHS 60

Query: 55  PLGSSKRS-GVAIRAMSSES---GPQGLPIDLRGKRAFIAGVADDNGYGWXXXXXXXXXX 110
            + + KR    + RAMS  S    P GLPIDLRGKRAFIAG+ADDNGYGW          
Sbjct: 61  AVPTCKRPFSFSTRAMSESSENKAPSGLPIDLRGKRAFIAGIADDNGYGWAIAKSLAAAG 120

Query: 111 XEILVGTWVPALNIFETSLRRGKFDESRKLPDGSLMEIVKVYPLDAVYDSPEDVPEDVKG 170
            EILVGTWVPALNIFETSLRRGKFD+SR LPDGSLMEI KVY LDAV+D+PEDVPEDVK 
Sbjct: 121 AEILVGTWVPALNIFETSLRRGKFDQSRVLPDGSLMEIKKVYALDAVFDNPEDVPEDVKT 180

Query: 171 NKRYAGSSNWTVKEVAESVKNDFGSIDILVHSLANGPEVTKPLLETSRRGYXXXXXXXXX 230
           NKRYAGSSNWTV+E AE VK DFGSIDILVHSLANGPEV+KPLLETSR+GY         
Sbjct: 181 NKRYAGSSNWTVQEAAECVKKDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSY 240

Query: 231 XXXXXXQHFLPIMNPGGASISLTYIASERAIPGYGGGMSSAKAALESDTKVLAFEAGRKG 290
                 +HFLPIMNPGGASISLTYIASER IPGYGGGMSSAKAALESDT+VLA+EAGRK 
Sbjct: 241 SFVSLLRHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKS 300

Query: 291 KIRVNTISAGPLGSRAAKAIGFIEKMIEYSYVNAPLQKELLADEVGNTAAFLVSPLASAI 350
            IRVNTISAGPLGSRAAKAIGFI+ MIEYSY N P+QK L ADEVGN AAFL SPLASAI
Sbjct: 301 NIRVNTISAGPLGSRAAKAIGFIDTMIEYSYNNGPIQKTLTADEVGNAAAFLASPLASAI 360

Query: 351 TGSTVYVDNGLNTMGLAVDSPTI 373
           TG+T+YVDNGLN MG+A+DSP  
Sbjct: 361 TGATIYVDNGLNAMGVALDSPVF 383



>AT1G07440.1 | chr1:2286436-2287665 REVERSE LENGTH=267
          Length = 266

 Score = 48.9 bits (115), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 48/188 (25%), Positives = 87/188 (46%), Gaps = 8/188 (4%)

Query: 183 KEVAESVKNDFG-SIDILVHSLANGPEVTKPLLETSRRGYXXXXXXXXXXXXXXXQHFLP 241
           +++ ++V + FG  +DIL+++L  G   +KP L+ +   +               Q   P
Sbjct: 79  EKLMQTVSSMFGGKLDILINNL--GAIRSKPTLDYTAEDFSFHISTNLESAYHLSQLAHP 136

Query: 242 IMNPGGASISLTYIASERAI--PGYGGGMSSAKAALESDTKVLAFEAGRKGKIRVNTISA 299
           ++   G   ++ +++S   +     G   S+ K AL    + LA E    G IR N ++ 
Sbjct: 137 LLKASGCG-NIIFMSSIAGVVSASVGSIYSATKGALNQLARNLACEWASDG-IRANAVAP 194

Query: 300 GPLGSRAAKAIGFIEKMIEYSYVNAPLQKELLADEVGNTAAFLVSPLASAITGSTVYVDN 359
             + +  A+A+ + ++  +      PL +    +EV +  AFL  P AS ITG T+ VD 
Sbjct: 195 AVIATPLAEAV-YDDEFKKVVISRKPLGRFGEPEEVSSLVAFLCMPAASYITGQTICVDG 253

Query: 360 GLNTMGLA 367
           GL   G +
Sbjct: 254 GLTVNGFS 261



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.131    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,047,717
Number of extensions: 275885
Number of successful extensions: 592
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 599
Number of HSP's successfully gapped: 6
Length of query: 375
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 275
Effective length of database: 8,364,969
Effective search space: 2300366475
Effective search space used: 2300366475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 112 (47.8 bits)