BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0323000 Os08g0323000|AK064573
         (418 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G61970.1  | chr5:24888920-24893079 FORWARD LENGTH=606          545   e-155

>AT5G61970.1 | chr5:24888920-24893079 FORWARD LENGTH=606
          Length = 605

 Score =  545 bits (1404), Expect = e-155,   Method: Compositional matrix adjust.
 Identities = 272/419 (64%), Positives = 329/419 (78%), Gaps = 9/419 (2%)

Query: 4   FKNTRAVYEELGKYGSIENQLLCRQRIEEVEPMIGFCSRKLGGSALQAHELLDL-EKEGP 62
           FKN RAVYEELGKYG +ENQ+LCR+R+EE+EP I +C  K+G S LQ  ELL + E EGP
Sbjct: 181 FKNARAVYEELGKYGDLENQVLCRERVEELEPSIRYCLHKIGKSNLQTSELLQIGEMEGP 240

Query: 63  AYDLFKAKIEAVLSETRSQQAASMTEFSWLGRRFPITNAKTRVSILKAQQLEKDLNGANT 122
           A DLFKAKIEA + E RSQQAAS+TEFSWLG RFP++N K+RVSILKAQ+LEK+L     
Sbjct: 241 ALDLFKAKIEAAMEEARSQQAASLTEFSWLGYRFPVSNPKSRVSILKAQELEKELQSPTA 300

Query: 123 ESVPADKKLGIFDKIFSAYHDARSCIRNDLASAGNAENIRDELNGLDKAVSAVLGFRTIE 182
           ES+PA+KKL IFDK+F+AYHDAR+ IR+DL SAGNAE+++D+LNGLDKAV AVLG RTIE
Sbjct: 301 ESLPAEKKLTIFDKLFTAYHDARNTIRSDLVSAGNAESVKDDLNGLDKAVGAVLGQRTIE 360

Query: 183 RNQLLVSIAKSKFTKHRDEKNEKITKPEELVRLYDLLIQNTTDLTDLISSGRDKNKEENT 242
           RNQLLV +AKSK  + RD+KN+K+T+PEELVRLYDLL+QN  DL+DLISSGRD+  EE  
Sbjct: 361 RNQLLVKVAKSKLNRKRDDKNDKVTRPEELVRLYDLLLQNVADLSDLISSGRDRKPEEIA 420

Query: 243 FIQEYELKSLAFRAERCFYLAKSYSSAGKRAESYALFCHARSITDSALQQLTN--SPDKA 300
           F +E +  SLAFRAERCFYLAKSYS AGKRAE++AL+  ARS+ + AL +  +    D+ 
Sbjct: 421 FEEECQRTSLAFRAERCFYLAKSYSLAGKRAEAFALYSRARSLAEDALNKFQSIAKKDEG 480

Query: 301 LVQDLKALSDSCRSNSFIEHATGIMEEENVPERLSKGVSTLSLGERKA-----FLLDMLE 355
            +Q+LK L   CR+NS IEHATGIMEEE  PE LS  +ST+SL    A     +LLD L+
Sbjct: 481 TIQELKTLIKECRANSCIEHATGIMEEERAPENLSNRMSTISLNNTAAQVEEKYLLDKLD 540

Query: 356 SYESALGESNTKTPCRIARFPPPFQSVPCNPIVLDMAYNAIEFPNIENRMKKEKKGLLS 414
            YESA+G+SNTKT  +I RFPP  QS+P NPIVLD+AYN I+FP IENRMKK K G  S
Sbjct: 541 VYESAVGDSNTKTAPKIERFPPALQSIPRNPIVLDLAYNCIDFPVIENRMKK-KGGFFS 598



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.131    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,562,203
Number of extensions: 346933
Number of successful extensions: 1036
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1033
Number of HSP's successfully gapped: 1
Length of query: 418
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 317
Effective length of database: 8,337,553
Effective search space: 2643004301
Effective search space used: 2643004301
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 113 (48.1 bits)