BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0320400 Os08g0320400|J065059G01
(103 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G04400.1 | chr2:1531208-1533578 FORWARD LENGTH=403 149 4e-37
AT5G48220.1 | chr5:19549930-19552046 FORWARD LENGTH=380 139 2e-34
>AT2G04400.1 | chr2:1531208-1533578 FORWARD LENGTH=403
Length = 402
Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust.
Identities = 73/104 (70%), Positives = 86/104 (82%), Gaps = 1/104 (0%)
Query: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLD-MHGDTIREKGILVVGESG 59
VHDEREM RVL I G++L+GINNRSLETF VD SNTK LL+ HG IRE+ ++VVGESG
Sbjct: 298 VHDEREMGRVLGIEGIELVGINNRSLETFEVDISNTKKLLEGEHGRQIRERDMIVVGESG 357
Query: 60 LFNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKELLH 103
LF PDD+AYVQ AGV AVLVGES+VKQ+DP + IAGLFG+ + H
Sbjct: 358 LFTPDDIAYVQAAGVKAVLVGESIVKQNDPEKGIAGLFGRNISH 401
>AT5G48220.1 | chr5:19549930-19552046 FORWARD LENGTH=380
Length = 379
Score = 139 bits (351), Expect = 2e-34, Method: Compositional matrix adjust.
Identities = 70/102 (68%), Positives = 85/102 (83%), Gaps = 1/102 (0%)
Query: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLD-MHGDTIREKGILVVGESG 59
VHDEREM+RVL I GV+LIGINNR+LETF VD TK LL+ G+ IR+K ILVVGESG
Sbjct: 276 VHDEREMDRVLAIEGVELIGINNRNLETFEVDLGITKKLLEGERGELIRQKDILVVGESG 335
Query: 60 LFNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKEL 101
LF P+D+A+VQ AGV AVLVGESL+KQ DPG+AI+ LFG+++
Sbjct: 336 LFTPEDIAFVQEAGVKAVLVGESLIKQSDPGKAISTLFGRDV 377
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.317 0.138 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,144,588
Number of extensions: 79314
Number of successful extensions: 188
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 186
Number of HSP's successfully gapped: 2
Length of query: 103
Length of database: 11,106,569
Length adjustment: 73
Effective length of query: 30
Effective length of database: 9,105,201
Effective search space: 273156030
Effective search space used: 273156030
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 104 (44.7 bits)