BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0299000 Os08g0299000|AK101640
         (298 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G25280.1  | chr2:10762639-10764631 REVERSE LENGTH=292          470   e-133

>AT2G25280.1 | chr2:10762639-10764631 REVERSE LENGTH=292
          Length = 291

 Score =  470 bits (1209), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 212/291 (72%), Positives = 253/291 (86%)

Query: 1   MERVRRASHAGSWYTNNARKLDEELDGWLRAAGLTKSPDVRAVIAPHAGYSYSGRCAAYA 60
           ME++R+ +HAGSWYT+N  KL  +L+ WL A GLTKSPDVR VIAPHAGYSYSGR AAYA
Sbjct: 1   MEKIRQPTHAGSWYTDNPTKLSSDLEEWLNATGLTKSPDVRGVIAPHAGYSYSGRAAAYA 60

Query: 61  FGNIDPTNISRVFLLGPSHHYYTPKCALTRATIYSTPIGDLPVDHEVIEELNATGKFDFM 120
           F NIDPTNISR+FLLGPSHH+YTPKCAL+ AT+Y TPIG+LPVD E+I+E+ A GKF  M
Sbjct: 61  FANIDPTNISRIFLLGPSHHFYTPKCALSTATVYKTPIGNLPVDVEMIKEIRAMGKFGMM 120

Query: 121 DLSVDEAEHSMEMHLPYLSKVFQGHNVKVVPILVGALNSQNEAMYGQLLSKYLDDPKNFF 180
           DL VDEAEHSMEMHLPYL+KVF+G+NVKVVPILVGA++ +NEAMYG+LL+KY+DDPKNFF
Sbjct: 121 DLRVDEAEHSMEMHLPYLAKVFEGNNVKVVPILVGAVSPENEAMYGELLAKYVDDPKNFF 180

Query: 181 SISSDFCHWGTRFSYTYYDKSHGAIHKSIEALDRMGMEIIETGNPDAFKQYLQEYENTIC 240
           S+SSDFCHWG+RF+Y +YD +HGAIHKSIEALD+ GM+IIETG+PDAFK+YL E+ENTIC
Sbjct: 181 SVSSDFCHWGSRFNYMHYDNTHGAIHKSIEALDKKGMDIIETGDPDAFKKYLLEFENTIC 240

Query: 241 GRHPISVFLSMLKHCLTKIKIGFVRYEQSSQCKSMRDXXXXXXXXXXKVDT 291
           GRHPIS+FL MLKH  +KIKI F+RYEQSSQC++MRD          K++T
Sbjct: 241 GRHPISIFLHMLKHSSSKIKINFLRYEQSSQCQTMRDSSVSYASAAAKLET 291



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.135    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,459,783
Number of extensions: 261617
Number of successful extensions: 546
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 546
Number of HSP's successfully gapped: 1
Length of query: 298
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 200
Effective length of database: 8,419,801
Effective search space: 1683960200
Effective search space used: 1683960200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)