BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0290100 Os08g0290100|AK073404
         (607 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G11030.2  | chr5:3490993-3495078 REVERSE LENGTH=603            330   1e-90

>AT5G11030.2 | chr5:3490993-3495078 REVERSE LENGTH=603
          Length = 602

 Score =  330 bits (847), Expect = 1e-90,   Method: Compositional matrix adjust.
 Identities = 209/546 (38%), Positives = 320/546 (58%), Gaps = 32/546 (5%)

Query: 73  MLREVHAFLSSPSSNQLAIDALSLELVKPVAKLGALMRNCWDIANAIIEFFVSNCNPRDM 132
           +L E+   LSSP  +Q  IDALS  L K  +K   +   C  +   I++ FV  CNPRDM
Sbjct: 76  VLDEILKVLSSPQMDQDVIDALSFHLPKVTSKFADISSRCLQLVEEIVDRFVEACNPRDM 135

Query: 133 LSILCEAVDAPLA-SNGSVYFVLLFKELAKVLVLIQRRHTEQVKVTLPAVLRVMNAVIPE 191
           LSILCEA+DA     + S     L   L+KV +LIQRRH EQ+KV +P VL V+  +  E
Sbjct: 136 LSILCEALDAARCYHSASTCSTPLLHGLSKVFILIQRRHYEQLKVAVPIVLNVLKDISLE 195

Query: 192 CDEEHGKIIVDMYNAALRIGNAIQEMCKKMVNQTNEELCSVLSLYSLQNIALVSRCKQQH 251
            D +    + D+++ AL I ++I+++  K+ N+   ++  +L LY +Q  A++S   +  
Sbjct: 196 TDVQ----VEDLFDKALGIASSIRDVSSKLNNEEEAKVRCLLCLYVIQITAIISVSIRDK 251

Query: 252 ILSACGSVVLQHSKILTFCGFTYLGLLTGNDVTSATDKISKDEDADLLECFSFAMNGANL 311
             ++C  +V+Q    LT CG T+LGL+TGND       ++ D+D + +  F     GA+L
Sbjct: 252 A-ASCIPLVIQLEPFLTSCGLTHLGLITGNDTEKLMSTVAGDDD-EFITSFPDISLGASL 309

Query: 312 AVIWTYMDDEISKYAGAELESALKDVKGNHTRMWQAINILRYVLSSTHYPWVIKSHSLDL 371
             I   +  E+++ A A L S + +++ N  + WQA  +L+Y+LSS    W  K H+++ 
Sbjct: 310 LFICAKISHEVAEAANAVLGSVVDELQNNPVKRWQAYGMLKYILSSGDLLWEFKRHAIEF 369

Query: 372 LLTIANESRIEEIND-HVDVSSSGPQIFATLKAIESVMISAPDALMRKKAFATLKQVISM 430
           LL I       + ND  +D S   P I+ATL+A+  +++ APDA +RKK F  LK+V+S 
Sbjct: 370 LLDITKGVTSSQCNDEQIDCSDYTPGIYATLQAVTLLIMYAPDADLRKKTFEALKRVLSD 429

Query: 431 VPSSQRFNILQALIKNSIFPSLTALLLDLVKDEVSREIRRADQDIVESDQLQDGGEWPPP 490
           +P+  RF++L+AL+ NS  PS+TA+LL LVKD +S+   + D D    D           
Sbjct: 430 IPAPHRFDVLRALVTNSRSPSMTAILLGLVKDSMSKSSLQ-DTDCAAVD----------- 477

Query: 491 WFSHALELVELILKPPEGGPPCLPDHGEQVLSALNLLRFVLIIDSRGSRSRK-------- 542
             +H +ELVEL+L+PP+GGPP LPD  + +L+ALNL RF L+ +SR   + K        
Sbjct: 478 --THVIELVELVLRPPQGGPPLLPDQSDAILAALNLYRFALLFESRECEAGKERSKVGSD 535

Query: 543 MFGEETMRKVYSEWLMPLRPIVAGIQSES--EEDGSDVANHIMCSLNPVQLVLYRCIELA 600
           +  ++ + K Y EWL+PLR +V+   +E+  E+ G + +   +  LNP++LVLYRCIEL 
Sbjct: 536 ILSKKNLEKAYKEWLLPLRTLVSCSIAENLKEDHGQESSLDDVGLLNPIELVLYRCIELV 595

Query: 601 EEKMKG 606
           EEK+K 
Sbjct: 596 EEKLKS 601



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,371,107
Number of extensions: 463691
Number of successful extensions: 1249
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1242
Number of HSP's successfully gapped: 1
Length of query: 607
Length of database: 11,106,569
Length adjustment: 104
Effective length of query: 503
Effective length of database: 8,255,305
Effective search space: 4152418415
Effective search space used: 4152418415
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 115 (48.9 bits)