BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0278900 Os08g0278900|AK100085
         (217 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G25110.1  | chr2:10684428-10685838 FORWARD LENGTH=219          316   7e-87

>AT2G25110.1 | chr2:10684428-10685838 FORWARD LENGTH=219
          Length = 218

 Score =  316 bits (809), Expect = 7e-87,   Method: Compositional matrix adjust.
 Identities = 144/215 (66%), Positives = 171/215 (79%)

Query: 1   MAAASFAIAXXXXXXXXXPEASPAQSYAADPDNVVEITYGSAIKLMHERTKFRLHSHDVP 60
           MA   F +A              + S AA     VEITYGSAIKLMHE+TKFRLHSHDVP
Sbjct: 1   MALGFFCLAIFLYLSLDPDSGYTSASAAASGKEGVEITYGSAIKLMHEKTKFRLHSHDVP 60

Query: 61  YGSGSGQQSVTSFPNVDDSNSYWIVRPQPDTSAKQGDPITHGTVVRLQHMRTRKWLHSHM 120
           YGSGSGQQSVT FP V DSNSYWIV+P P T+ KQGD +  G  +RLQHM+TRKWLHSH+
Sbjct: 61  YGSGSGQQSVTGFPGVVDSNSYWIVKPVPGTTEKQGDAVKSGATIRLQHMKTRKWLHSHL 120

Query: 121 HASPITGNLEVSCFGGENESDTGDYWRLEIEGSGKSWRQNQKIRLRHVDTGGYLHSHDRK 180
           HASPI+GNLEVSCFG +  SDTGD+W+L IEGSGK+W+Q+Q++RL+H+DT GYLHSHD+K
Sbjct: 121 HASPISGNLEVSCFGDDTNSDTGDHWKLIIEGSGKTWKQDQRVRLQHIDTSGYLHSHDKK 180

Query: 181 YTRIAGGQQEVCGVGDKRPDNVWLAAEGVYLPVNQ 215
           Y RIAGGQQEVCG+ +K+ DN+WLAAEGVYLP+N+
Sbjct: 181 YQRIAGGQQEVCGIREKKADNIWLAAEGVYLPLNE 215



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.131    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,237,457
Number of extensions: 239166
Number of successful extensions: 461
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 455
Number of HSP's successfully gapped: 1
Length of query: 217
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 123
Effective length of database: 8,529,465
Effective search space: 1049124195
Effective search space used: 1049124195
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 109 (46.6 bits)