BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0266300 Os08g0266300|Os08g0266300
(339 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G16080.1 | chr1:5514394-5515761 FORWARD LENGTH=314 313 7e-86
>AT1G16080.1 | chr1:5514394-5515761 FORWARD LENGTH=314
Length = 313
Score = 313 bits (803), Expect = 7e-86, Method: Compositional matrix adjust.
Identities = 163/280 (58%), Positives = 186/280 (66%), Gaps = 50/280 (17%)
Query: 58 QALLSMGPPGADVLVGRGEKVPEDAPGPILVCTRNDDLDAVLDATPRSRWRGTFVXXXXX 117
+AL MG G D+LV RGE VP D GPILVCTRNDDLDAVL+ATP+SRW+
Sbjct: 67 RALQEMGN-GEDLLVKRGEAVPVDFEGPILVCTRNDDLDAVLEATPQSRWKDLVF----- 120
Query: 118 XXXXXXXXXXXXXXXXXXXXXXXXXXXIQNGMLDPWFESKGLVDANQVLAYFAVSKLGEP 177
QNGM++PWFESKGL D +QVLAYFAVSKLGEP
Sbjct: 121 ---------------------------FQNGMMEPWFESKGLGDTDQVLAYFAVSKLGEP 153
Query: 178 PVDGITDTNPEGLTAAFGNWAPAVATRLQNGGLTCKVLEKEAFQKQMLEKLIWISAFMLV 237
PVDG TDTNPEGLTAA+G WA +A RLQ+GGL+CKVL+KEAFQKQMLEKLIWI AFMLV
Sbjct: 154 PVDGKTDTNPEGLTAAYGKWASEIAARLQSGGLSCKVLDKEAFQKQMLEKLIWICAFMLV 213
Query: 238 GARHPGATVGAVEKEYQXXXXXXXXXXXXXXXXXRGLTFDAGIEERLCAYSRAVAHFPTA 297
GARHPGA+VG VEKEY+ +GLTF+ + ERLCAYSRAV+HFPTA
Sbjct: 214 GARHPGASVGTVEKEYRDEVSRLIQELAAAAAAEKGLTFEENMVERLCAYSRAVSHFPTA 273
Query: 298 -----------------ALAQGKPDPCPLHTAWLKEIKVI 320
A+A+G+PDPCPLHT WLKE+KVI
Sbjct: 274 VKEFKWRNGWFYSLSEKAIAEGQPDPCPLHTEWLKELKVI 313
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.320 0.136 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,622,391
Number of extensions: 190472
Number of successful extensions: 318
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 316
Number of HSP's successfully gapped: 2
Length of query: 339
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 240
Effective length of database: 8,392,385
Effective search space: 2014172400
Effective search space used: 2014172400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)