BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0162500 Os08g0162500|AK121633
         (170 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G19350.1  | chr4:10562214-10563676 REVERSE LENGTH=181          119   1e-27

>AT4G19350.1 | chr4:10562214-10563676 REVERSE LENGTH=181
          Length = 180

 Score =  119 bits (297), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 65/160 (40%), Positives = 98/160 (61%), Gaps = 1/160 (0%)

Query: 11  RYGNLERSFKLAARSVLTACSREDVKRAFPSFTDAERERLYQMFIYVIKSLHSNIEEEFD 70
           R  +L++SFK   R +LTACS++D    F  F+ AE+E L+Q++  V+ +LH  IEEEFD
Sbjct: 14  RRTHLKKSFKSTLRHLLTACSKQDFVDIFSKFSGAEQELLFQLYTRVVVNLHQTIEEEFD 73

Query: 71  IPCQELQVAAALEKIDQFVEEQKLDVLSSDKTNIEDTKQMISKAKKDEVEYLKSLIEEVE 130
             C E QV   L+ +++ VEEQ LD L SDKT++      ++ +KK+E++ L  L++  E
Sbjct: 74  EQCHETQVGPILDTVEELVEEQSLDPLFSDKTDVMAIAYDLTTSKKNEIQKLTVLLQRAE 133

Query: 131 EKNNAMKARIELLKKD-DDLAAGKQVLEKLMQCNSALYNG 169
           E+N   +ARI +LKK   DL+     +EKL    S  + G
Sbjct: 134 EQNRQKEARISMLKKQTQDLSGTADRIEKLNAGVSGYFEG 173



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.130    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,186,046
Number of extensions: 127192
Number of successful extensions: 909
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 911
Number of HSP's successfully gapped: 1
Length of query: 170
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 79
Effective length of database: 8,611,713
Effective search space: 680325327
Effective search space used: 680325327
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 108 (46.2 bits)