BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0152500 Os08g0152500|AK060448
         (268 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G39170.1  | chr2:16334726-16335421 REVERSE LENGTH=232          146   9e-36

>AT2G39170.1 | chr2:16334726-16335421 REVERSE LENGTH=232
          Length = 231

 Score =  146 bits (369), Expect = 9e-36,   Method: Compositional matrix adjust.
 Identities = 78/156 (50%), Positives = 103/156 (66%), Gaps = 1/156 (0%)

Query: 22  DGFLCVKDGVDEMIKYVANEPSVGLYFVQQHAQASMPLLLDVKGKVAEKIHEVTLHTEDI 81
           DGF  + + + EMIKY+ANEPSVGLY++QQH + + P ++++   V EK  E  LHTED+
Sbjct: 8   DGFAEINESLAEMIKYIANEPSVGLYYIQQHIRNAAPNVINLNNNVLEKSRETGLHTEDL 67

Query: 82  EDSICAVRSMAEFGLPIADDMIKDINKSLKIMSKTQPKRGLIQNPTWGFQSGKSSGTWEE 141
           EDSI  V+SM + G PIAD+MI DI  SL IMS  QP+RG+I N T  +    S  T   
Sbjct: 68  EDSIAMVKSMKDCGSPIADEMIGDIKNSLAIMSSKQPRRGVILNSTSPWSRSSSITTRTR 127

Query: 142 DL-GTTDGGSSRNYFSSMFNTAKQKASTLRWPQPDF 176
               + D   S NYF+S+F TAK+KAS ++WPQ DF
Sbjct: 128 GSDNSQDNSESSNYFTSVFMTAKEKASNIKWPQLDF 163



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.127    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,115,199
Number of extensions: 194649
Number of successful extensions: 404
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 403
Number of HSP's successfully gapped: 1
Length of query: 268
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 171
Effective length of database: 8,447,217
Effective search space: 1444474107
Effective search space used: 1444474107
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 111 (47.4 bits)