BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0129500 Os08g0129500|AK122021
         (358 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G32730.1  | chr1:11841077-11842956 FORWARD LENGTH=328          191   7e-49

>AT1G32730.1 | chr1:11841077-11842956 FORWARD LENGTH=328
          Length = 327

 Score =  191 bits (484), Expect = 7e-49,   Method: Compositional matrix adjust.
 Identities = 119/330 (36%), Positives = 177/330 (53%), Gaps = 25/330 (7%)

Query: 38  SAFDMPKPNLRGLNKPKCIQCGNVARSRCPFQCCKSCCYKAQNPCHIHVLKQNNTLPDKP 97
           S+   P   LRGLNKPKCIQCGNVARSRCPFQ CK CC +A+NPC IHVLK  +T  +K 
Sbjct: 14  SSVVTPPKTLRGLNKPKCIQCGNVARSRCPFQSCKGCCSRAENPCPIHVLKVASTSGEKT 73

Query: 98  PLSTAPLSEQPSANIPSTGSSRLASLQRLPHHF-----LN-SLRTKKSLAKKDVMGINKW 151
              + P SEQ +       ++R++S+++L  +F     LN S R +K L  KD   +N+W
Sbjct: 74  QAPSTPSSEQKATEGTPGSTTRVSSIRQLSSNFAQFNNLNASSRQRKPLTIKDAQALNEW 133

Query: 152 RFEKLKEHIQGDIDAENEAYERYTQNVGLLEETFGLXXXXXXXXXXXXXXXXXRMETLVS 211
           RF KLKE+   +I+ ENEA++RY  NV LLEE F                   + E +VS
Sbjct: 134 RFTKLKEYRDRNIEVENEAFDRYMSNVNLLEEAFSFTSVPDEESHGTAAPEQNKEENIVS 193

Query: 212 EAKVRLKSDSANADGFRDRIATILDQ---KIKELLESKSTYEXXXXXXXXXXXXXXXVKL 268
           E K+RL+S+SA  + F+ RIA  +     K+K L    S+ +                ++
Sbjct: 194 ELKLRLRSNSARTESFKKRIAETVKAGLVKLKRLDLGSSSDDQDDIKR----------RV 243

Query: 269 SIKQQMERGAKMTELLAKMIRAQSEVDLKICSGIAAQLFGKKNELSNQESVASVTIPYSF 328
             K+  E+G+ + E++ K+ +A++E DLK C  + ++L G+ +  +  E          F
Sbjct: 244 KRKKWEEKGSALNEIIDKLNKARTEEDLKSCLEMKSKLCGQVSPTAASEK------NKIF 297

Query: 329 PKLWTRVEIDDAMMCKINGEFSSSTEVMQL 358
           P +  +VE+ +  + KI     S  +V  L
Sbjct: 298 PGVVRKVEMSEEALQKIAENLQSFDKVGML 327



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.130    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,404,518
Number of extensions: 243385
Number of successful extensions: 904
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 902
Number of HSP's successfully gapped: 1
Length of query: 358
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 258
Effective length of database: 8,364,969
Effective search space: 2158162002
Effective search space used: 2158162002
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 112 (47.8 bits)