BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0675400 Os07g0675400|AK065735
         (286 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G28350.1  | chr1:9944483-9949577 FORWARD LENGTH=749            246   1e-65
AT2G33840.1  | chr2:14315011-14317329 FORWARD LENGTH=386          245   2e-65

>AT1G28350.1 | chr1:9944483-9949577 FORWARD LENGTH=749
          Length = 748

 Score =  246 bits (627), Expect = 1e-65,   Method: Compositional matrix adjust.
 Identities = 122/260 (46%), Positives = 169/260 (65%), Gaps = 12/260 (4%)

Query: 20  YNIEMWKATGMALDKVDLVWLSDEIRQHGDEYWPIVMDIARTNSVRRIKSTCRSSYYCLP 79
           Y  E+++A GM  + V+ +W SDEI   GDEYWP+VMDIA  NS+ +IK        C+P
Sbjct: 117 YFKEIFQAAGMNSENVEFLWSSDEINAKGDEYWPLVMDIACRNSLAQIKR-------CMP 169

Query: 80  SRFCGSRNSYAIGKLNVDEIFYICLQCASILFQKADIWLLGMEQHDANLLARQYYKHFKK 139
                        +L+   I Y+C+QCA   F +ADI  LGM+Q   NLLAR Y    K+
Sbjct: 170 IMGLSENE-----ELSAAHILYVCMQCADTFFLEADICQLGMDQQTVNLLARDYCDVVKR 224

Query: 140 KNKAIAVLDNMLPNLLQYPQMENRRHPAWAIFMEDDKEDICFKMEKAFCPPKLAEGNPCL 199
           +NK + +  +MLP L Q  +  ++  P+ AIFMED++ ++  K++KA+CPP + EGNPCL
Sbjct: 225 ENKPVILSHHMLPGLQQGQKKMSKSDPSSAIFMEDEEAEVNVKIKKAYCPPDIVEGNPCL 284

Query: 200 EYIKYIILPWFGMFEVVQKKGNGGKKTFLSMEELTADYESGALHPADVKLALEKSLNEIL 259
           EY+K+IILPWF  F V + +  GG +TF S E++T DYESG LHP D+K AL K+LN+IL
Sbjct: 285 EYVKHIILPWFSEFTVERDEKYGGNRTFKSFEDITTDYESGQLHPKDLKDALSKALNKIL 344

Query: 260 QPVRDHFGRNGEAKDLVEAI 279
           QPVRDHF  N  AK+L++ +
Sbjct: 345 QPVRDHFKTNSRAKNLLKQV 364




 Score =  234 bits (597), Expect = 4e-62,   Method: Compositional matrix adjust.
 Identities = 129/263 (49%), Positives = 170/263 (64%), Gaps = 19/263 (7%)

Query: 20  YNIEMWKATGMALDKVDLVWLSDEIRQHGDEYWPIVMDIARTNSVRRIKSTCRSSYYCLP 79
           Y  E+W+A GM  DKV+ +W SDEI   G +YWP+VMDIAR N++RRI            
Sbjct: 495 YFKEIWQAGGMNNDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILR---------- 544

Query: 80  SRFCGS---RNSYAIGKLNVDEIFYICLQCASILFQKADIWLLGMEQHDANLLARQYYKH 136
              CG    R+   +  L+  +I Y C+QCA I   +ADI  LGM+Q   N+LAR+Y   
Sbjct: 545 ---CGQIMGRSETEV--LSAAQILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCAD 599

Query: 137 FKKKNKAIAVLDNMLPNLLQYPQMENRRHPAWAIFMEDDKEDICFKMEKAFCPPKLAEGN 196
            K+KNK I +  +MLP L Q  +  ++  P+ AIFMED++ D+  K+ KA+CPPK  EGN
Sbjct: 600 IKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGN 659

Query: 197 PCLEYIKYIILPWFGMFEVVQKKGNGGKKTFLSMEELTADYESGALHPADVKLALEKSLN 256
           PCLEY+KYI+LP F  F+V  +K NGG KTF S E++ ADYE+G LHP D+K AL K+LN
Sbjct: 660 PCLEYVKYIVLPRFNEFKVESEK-NGGNKTFNSFEDIVADYETGELHPEDLKKALMKALN 718

Query: 257 EILQPVRDHFGRNGEAKDLVEAI 279
             LQPVRDHF  N  AK+L+E +
Sbjct: 719 ITLQPVRDHFKTNERAKNLLEQV 741



>AT2G33840.1 | chr2:14315011-14317329 FORWARD LENGTH=386
          Length = 385

 Score =  245 bits (625), Expect = 2e-65,   Method: Compositional matrix adjust.
 Identities = 125/260 (48%), Positives = 170/260 (65%), Gaps = 12/260 (4%)

Query: 20  YNIEMWKATGMALDKVDLVWLSDEIRQHGDEYWPIVMDIARTNSVRRIKSTCRSSYYCLP 79
           Y  E+WKA GM  DKV+ +W S+EI    D+YWP+VMDIAR N + RI         C+ 
Sbjct: 131 YFQEIWKAAGMDNDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRI-------LRCV- 182

Query: 80  SRFCGSRNSYAIGKLNVDEIFYICLQCASILFQKADIWLLGMEQHDANLLARQYYKHFKK 139
            +  G   +    +L+  +I Y C+QCA I F +ADI  LGM+Q   N+LAR+Y    K+
Sbjct: 183 -QIMGRSET---DELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKR 238

Query: 140 KNKAIAVLDNMLPNLLQYPQMENRRHPAWAIFMEDDKEDICFKMEKAFCPPKLAEGNPCL 199
           KNK I +  +MLP L Q  +  ++  P  AIFMED++ ++  K++KA+CPPK+ +GNPCL
Sbjct: 239 KNKPIILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCL 298

Query: 200 EYIKYIILPWFGMFEVVQKKGNGGKKTFLSMEELTADYESGALHPADVKLALEKSLNEIL 259
           EYIKYIILPWF  F V + +  GG KT+ S E++ ADYESG LHP D+K  L  +LN+IL
Sbjct: 299 EYIKYIILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKIL 358

Query: 260 QPVRDHFGRNGEAKDLVEAI 279
           QPVRDHF  +  AK+L++ I
Sbjct: 359 QPVRDHFKTDARAKNLLKQI 378



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.139    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,818,502
Number of extensions: 289605
Number of successful extensions: 588
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 584
Number of HSP's successfully gapped: 3
Length of query: 286
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 188
Effective length of database: 8,419,801
Effective search space: 1582922588
Effective search space used: 1582922588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 111 (47.4 bits)