BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0644000 Os07g0644000|AK064653
         (321 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G40660.1  | chr5:16283650-16285161 REVERSE LENGTH=326          370   e-103

>AT5G40660.1 | chr5:16283650-16285161 REVERSE LENGTH=326
          Length = 325

 Score =  370 bits (950), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 179/269 (66%), Positives = 212/269 (78%), Gaps = 3/269 (1%)

Query: 53  KKPAAAAAVTTR---DETSVAMPMSFMTGSVVGKRFYREATVRRADDGNGWSVMLDYRTL 109
           +KP    A  +R   +  SV MP SFMTGS+VGKRFY++ T R ADDGNGW+VMLDYRTL
Sbjct: 54  EKPILVKAPNSRRKNESDSVTMPTSFMTGSIVGKRFYKKVTTREADDGNGWTVMLDYRTL 113

Query: 110 KSPAKRPLKLQSRTLAMAIAAEWEYQEADGIRPFTMPLMKLACTALERVPLTRKKIIDNL 169
           K+P+KRPLKL+S  LA AIAAEWEYQ  +GIRPFTMPLM+LACTALERVPLTR KII++L
Sbjct: 114 KTPSKRPLKLRSLALAKAIAAEWEYQLTEGIRPFTMPLMRLACTALERVPLTRSKIIEHL 173

Query: 170 MKKFHQDLVFCRSPDDNELTVGVYQRQKEKIDPILDWVNTEFGFKPVVYTSFFGGKQDEG 229
            +K HQDLVF R+P+DN+LT  V+  Q E IDP+L+W+ +EF  KP VY+S FGGKQD+ 
Sbjct: 174 SRKIHQDLVFFRAPEDNDLTSDVHDIQVESIDPLLEWIESEFRVKPKVYSSIFGGKQDDK 233

Query: 230 LANAVETVLKNTTDFELASIDXXXXXXHSLVIPLAIFRGKLGIEQAIELIRLEEDHQVDR 289
           L  AVE +LK T D ELASID      HS+VI L IF GKL I+ AI+LIRLEED QVD+
Sbjct: 234 LVKAVEELLKKTNDGELASIDALQASAHSIVIALGIFCGKLQIDDAIKLIRLEEDLQVDK 293

Query: 290 WGLVEGGHDVDIADLKVQMSSAVVFLLLS 318
           WGLVEGGHD+D+ADLKVQ+SSA VFL LS
Sbjct: 294 WGLVEGGHDIDVADLKVQISSATVFLALS 322



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,660,591
Number of extensions: 225221
Number of successful extensions: 451
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 451
Number of HSP's successfully gapped: 1
Length of query: 321
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 222
Effective length of database: 8,392,385
Effective search space: 1863109470
Effective search space used: 1863109470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)