BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0640100 Os07g0640100|AK070897
(131 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G12260.1 | chr3:3909252-3910337 REVERSE LENGTH=134 191 7e-50
>AT3G12260.1 | chr3:3909252-3910337 REVERSE LENGTH=134
Length = 133
Score = 191 bits (486), Expect = 7e-50, Method: Compositional matrix adjust.
Identities = 89/129 (68%), Positives = 107/129 (82%), Gaps = 2/129 (1%)
Query: 3 FTMRAVKVPPNSASLEEARHRVFDFFRQACRAIPSIMEIYNLDDVVTPSQLRSTIAKEIR 62
F +R + VPPNSA+L EAR RVFDFFR ACR+IP+IM+IYNL DVV PSQLR I+ +IR
Sbjct: 5 FALRKIGVPPNSANLTEARRRVFDFFRAACRSIPTIMDIYNLQDVVAPSQLRYAISAQIR 64
Query: 63 KNQGVTNPKVIDMLLFKGMEELGNITEHAKQRHHVIGQYVVGQKGLVQDM-EKDQGSSDF 121
N +T+PKVID+L+FKGMEEL +I +HAKQRHH+IGQYVVG+ GLVQ+ KDQG +DF
Sbjct: 65 NNAHITDPKVIDLLIFKGMEELTDIVDHAKQRHHIIGQYVVGE-GLVQNTGNKDQGKTDF 123
Query: 122 LKKFYTSNY 130
LK FYTSNY
Sbjct: 124 LKNFYTSNY 132
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.320 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,730,413
Number of extensions: 101022
Number of successful extensions: 226
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 226
Number of HSP's successfully gapped: 1
Length of query: 131
Length of database: 11,106,569
Length adjustment: 87
Effective length of query: 44
Effective length of database: 8,721,377
Effective search space: 383740588
Effective search space used: 383740588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 106 (45.4 bits)