BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0466200 Os07g0466200|AK100328
         (484 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G61450.1  | chr5:24710240-24712882 REVERSE LENGTH=448          499   e-141

>AT5G61450.1 | chr5:24710240-24712882 REVERSE LENGTH=448
          Length = 447

 Score =  499 bits (1285), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 251/408 (61%), Positives = 295/408 (72%), Gaps = 25/408 (6%)

Query: 72  PSPSRNASSKYDFVKVKVWLGENADHYYVLSRFLLCRMLTVTKIPNHVXXXXXXXXXXXX 131
           PS  RNASSKYDFVKVKVWLG+NADHYYVLSRFL+CRMLTVTKIPNH             
Sbjct: 42  PSSPRNASSKYDFVKVKVWLGDNADHYYVLSRFLVCRMLTVTKIPNHEAIKISLELKKLL 101

Query: 132 VDNSLLDVSQSDLEANLFKLMEKRGYGEDYINRYKMMTRFHHQRVPLVVLVCGTACTGKS 191
           +DNSLLDVSQSDLE NLFKLME+RGYGE+YINRY MMT+FHHQRVPLV+LVCGTAC GKS
Sbjct: 102 IDNSLLDVSQSDLETNLFKLMERRGYGEEYINRYNMMTKFHHQRVPLVILVCGTACVGKS 161

Query: 192 TIATQLAGRLNLPNVLQTDMVYELLRTSTDAPLTSVPVWARDFDSPEELITEFCRECRVV 251
           TIATQLA RLNLPNVLQTDMVYELLRT+TDAPLTS PVW R+F S EELITEFCRECR+V
Sbjct: 162 TIATQLAQRLNLPNVLQTDMVYELLRTATDAPLTSTPVWTREFGSSEELITEFCRECRIV 221

Query: 252 RKGLAGDLKKAMKDGKPIIIEGIHLDPSIYFMDEEKRDDNSKMEKKVAECEQS------- 304
           RKGL GDLKKAMKDGKPIIIEG HLDPSIY M++E +  ++  EK  +E   S       
Sbjct: 222 RKGLGGDLKKAMKDGKPIIIEGRHLDPSIYLMNDENKTPSNDPEKSSSETNSSKDLTSDK 281

Query: 305 -PASVESKTERQQENELHEKRMDDSQECMSEEGGISEGLSCAKSHVISSSDSAYSKEKNP 363
            P +  S T ++ +N   +    +      E   +SE ++       SS D   +++K+ 
Sbjct: 282 NPEASSSNT-KETDNSAVKPHSHEEAVASVEANNLSEKVTQCTIDAESSKD---AEKKSK 337

Query: 364 RAEDEGHKDLDLQKNNTTKKDKPAAEPIVVPIVLRMSDFDHKALLEEWTATRASRDNCLP 423
            A+  G             K K   EPIV+ IVL+MS+FDHKALLEEW ++R S +    
Sbjct: 338 SADGSG-------------KTKSGPEPIVISIVLKMSEFDHKALLEEWISSRTSGEKYTT 384

Query: 424 QDHRKLINNLKLIQDYLCSFESQGLTVVDISANSFPQTLDWLHSYLLQ 471
           ++  +LI NLK I+DYLCSF SQG+TVV+ISA +FPQTLDWLH+YLLQ
Sbjct: 385 KEKDRLITNLKTIEDYLCSFNSQGVTVVNISATTFPQTLDWLHNYLLQ 432



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.132    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 10,612,327
Number of extensions: 440733
Number of successful extensions: 2109
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 2113
Number of HSP's successfully gapped: 1
Length of query: 484
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 382
Effective length of database: 8,310,137
Effective search space: 3174472334
Effective search space used: 3174472334
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 114 (48.5 bits)