BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0250900 Os07g0250900|AK107935
         (227 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G35980.1  | chr2:15110635-15111318 FORWARD LENGTH=228           52   3e-07

>AT2G35980.1 | chr2:15110635-15111318 FORWARD LENGTH=228
          Length = 227

 Score = 51.6 bits (122), Expect = 3e-07,   Method: Compositional matrix adjust.
 Identities = 39/126 (30%), Positives = 62/126 (49%), Gaps = 8/126 (6%)

Query: 61  RHVSITVESASLTRFNLSSPSEATALAYNLSLTLAVRNKNWAMSIKNTKDLEAGYSFDGQ 120
           R +   V  ASLTRF+ +SP     L YNL+LT+ VRN N  + +   + +EA   ++G+
Sbjct: 66  RAIKFHVTDASLTRFDHTSPDNI--LRYNLALTVPVRNPNKRIGLYYDR-IEAHAYYEGK 122

Query: 121 RFERVKLAGEGEKHPAGKTRVYHLDSSSDNAYAALGNAGVAE-FKKENATGVFEVEVAVT 179
           RF  + L    + H    T V  L  +       + NAG +     E  +GV+ +E+   
Sbjct: 123 RFSTITLTPFYQGH--KNTTV--LTPTFQGQNLVIFNAGQSRTLNAERISGVYNIEIKFR 178

Query: 180 GEVRYQ 185
             VR++
Sbjct: 179 LRVRFK 184



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.129    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,574,113
Number of extensions: 132661
Number of successful extensions: 283
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 286
Number of HSP's successfully gapped: 1
Length of query: 227
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 132
Effective length of database: 8,502,049
Effective search space: 1122270468
Effective search space used: 1122270468
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)