BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os06g0644800 Os06g0644800|AK109442
(298 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G35080.1 | chr5:13354552-13356725 REVERSE LENGTH=283 243 9e-65
>AT5G35080.1 | chr5:13354552-13356725 REVERSE LENGTH=283
Length = 282
Score = 243 bits (620), Expect = 9e-65, Method: Compositional matrix adjust.
Identities = 124/248 (50%), Positives = 167/248 (67%), Gaps = 11/248 (4%)
Query: 27 DQIFTSS-GAPFGRNSREPRYHVEFHPVDAPFNPENGQESVPMTSHVGKHYTCFLPVEET 85
DQIF + F RN+REP+Y +EF P D+PF+P + ES+ M G + C+LP EE
Sbjct: 24 DQIFPAHLVGTFSRNNREPKYKIEFLPEDSPFHPGDNLESMVMLDKHGNRFLCYLPKEEK 83
Query: 86 KTMK-SIIPQNATNVIIESERRVKPKDPDELLEILKDQCFYRHEGWWSYEFCYYGKIRQV 144
T + QN + V++E+++ VK K PDELL+ L ++C +R EGWWSYEFC+ +RQ+
Sbjct: 84 ATSGWTSSQQNISTVMMETQQLVKLKTPDELLQPLSEKCLFRQEGWWSYEFCHQKYVRQL 143
Query: 145 HVEGE-KVIQEYVLGEYDADATDAYYENQTSDSADEDDNLIDTSKRYHVHLYTNGTVCDL 203
HVE E K++QE+ LG +D +AT A+ NQT A D S+RYH H+YTNGT CDL
Sbjct: 144 HVEDENKIVQEFFLGTFDPEATAAF--NQTVSDAS-----TDASQRYHSHVYTNGTTCDL 196
Query: 204 TDMPRETEVRFVCSEPTVVISSIKEISSCKYVLTVQSPMLCKNPLFQQEK-RTLSIHCNE 262
T PRE EVRFVC+E +++SI E+S+CKY LTVQ P LCK+PLFQ EK + +IHCN
Sbjct: 197 TGSPREVEVRFVCAETRAMVTSITELSTCKYALTVQCPTLCKHPLFQLEKPVSHTIHCNA 256
Query: 263 LLAEAEAT 270
+ E +AT
Sbjct: 257 IPVEEDAT 264
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.316 0.133 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,546,124
Number of extensions: 287232
Number of successful extensions: 697
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 693
Number of HSP's successfully gapped: 1
Length of query: 298
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 200
Effective length of database: 8,419,801
Effective search space: 1683960200
Effective search space used: 1683960200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 111 (47.4 bits)