BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0233400 Os06g0233400|AK061460
         (250 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G12760.1  | chr3:4054963-4056826 FORWARD LENGTH=251            385   e-107
AT1G15860.3  | chr1:5455055-5456741 FORWARD LENGTH=237            113   8e-26
AT3G28970.1  | chr3:10987970-10989782 REVERSE LENGTH=296           92   3e-19

>AT3G12760.1 | chr3:4054963-4056826 FORWARD LENGTH=251
          Length = 250

 Score =  385 bits (989), Expect = e-107,   Method: Compositional matrix adjust.
 Identities = 179/249 (71%), Positives = 208/249 (83%), Gaps = 2/249 (0%)

Query: 1   MHKLGRGSRDKVQQFMTITGASEKVALQALKASDWHLEGAFDFFYSQPQ--ISLTNSRHL 58
           MHKL R +RDK+QQF+ ITGASEK ALQALKASDWHLE AFD FYSQPQ   +    R L
Sbjct: 1   MHKLSRSNRDKLQQFVAITGASEKNALQALKASDWHLEAAFDVFYSQPQPRSNAAEVRRL 60

Query: 59  EDLYNRYKEPDVDMIMVEGVSQFCTDLQVDPQDIVMLVISWHMKAATMCEFTRQEFIGGL 118
           E+LYNRYK+P  DMI+ EG+S  C DL+V+PQDIV LV+SWHM AAT CEF+RQEFI GL
Sbjct: 61  EELYNRYKDPYSDMILAEGISVLCNDLEVEPQDIVTLVLSWHMNAATACEFSRQEFISGL 120

Query: 119 QSIGVDSIEKLREKLPSLRAEIKDDHKFREIYNFAFAWAREKGQKSLALETALGMWQLLF 178
           Q++GVDSI KL+EKLP +R+E+KD+ KF EIYNFAF WA+EKGQKSLAL+TA+GMWQLLF
Sbjct: 121 QALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGWAKEKGQKSLALDTAIGMWQLLF 180

Query: 179 AERHWPLIDHWCQFLQVRHNKAISRDTWSQLLEFVKTIDPQLSNYDEEGAWPYLIDEFVE 238
           AER WPL+ HWC FLQ RHNKAIS+DTW+QLLEF + +DP LSNYD EGAWPYLIDEFVE
Sbjct: 181 AEREWPLVTHWCDFLQDRHNKAISKDTWAQLLEFSRMVDPVLSNYDAEGAWPYLIDEFVE 240

Query: 239 YLTENGFVQ 247
           YL +   V+
Sbjct: 241 YLYDKNVVE 249



>AT1G15860.3 | chr1:5455055-5456741 FORWARD LENGTH=237
          Length = 236

 Score =  113 bits (283), Expect = 8e-26,   Method: Compositional matrix adjust.
 Identities = 60/197 (30%), Positives = 108/197 (54%), Gaps = 12/197 (6%)

Query: 58  LEDLYNRYKEPDVDMIMVEGVSQFCTDLQVDPQDIVMLVISWHMKAATMCEFTRQEFIGG 117
           ++ L+N+Y      +I  EG+ + C++L+V   DI +L+++W MKA     FT +E+  G
Sbjct: 37  IDHLFNQYANKSSSLIDPEGIEELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRG 96

Query: 118 LQSIGVDSIEKLREKLPSLRAEIKDDHKFREIYNFAFAWA-REKGQKSLALETALGMWQL 176
           L+++  D+I KL++ LP L  E++    F + Y +AF +   E+ QKS+ +ET   + ++
Sbjct: 97  LKALRADTINKLKKALPELEKEVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEI 156

Query: 177 LFAERHWPLIDHWCQFLQV----------RHNKAISRDTWSQLLEFVKTID-PQLSNYDE 225
           +        +D++ ++L+V             K I+ D W  L  F   I  P + +Y+ 
Sbjct: 157 VMGSTFRAQVDYFVEYLKVWITQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNP 216

Query: 226 EGAWPYLIDEFVEYLTE 242
           E AWP ++D FVE++ E
Sbjct: 217 ELAWPLILDNFVEWIQE 233



>AT3G28970.1 | chr3:10987970-10989782 REVERSE LENGTH=296
          Length = 295

 Score = 91.7 bits (226), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 41/99 (41%), Positives = 60/99 (60%), Gaps = 1/99 (1%)

Query: 142 DDHKFREIYNFAFAWAREKGQKSLALETALGMWQLLFAERHWPLIDHWCQFLQVRHNKAI 201
           D  +F   Y+F F   RE GQK++ +  A+  W+L+ A R + L++ WC F++      I
Sbjct: 82  DFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGR-FRLLNRWCDFIEKNQRHNI 140

Query: 202 SRDTWSQLLEFVKTIDPQLSNYDEEGAWPYLIDEFVEYL 240
           S DTW Q+L F + +   L  YD EGAWP LID+FVE++
Sbjct: 141 SEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHM 179



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,217,025
Number of extensions: 205737
Number of successful extensions: 560
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 556
Number of HSP's successfully gapped: 3
Length of query: 250
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 154
Effective length of database: 8,474,633
Effective search space: 1305093482
Effective search space used: 1305093482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 110 (47.0 bits)