BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0596200 Os05g0596200|AK107887
(101 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G11600.1 | chr5:3733815-3735015 REVERSE LENGTH=243 124 1e-29
AT1G19990.1 | chr1:6935327-6936665 FORWARD LENGTH=252 84 1e-17
>AT5G11600.1 | chr5:3733815-3735015 REVERSE LENGTH=243
Length = 242
Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust.
Identities = 63/104 (60%), Positives = 78/104 (75%), Gaps = 10/104 (9%)
Query: 2 SSSSAAALARGKSGISAAKFEASKGLGAKPKRSASATGS----RGKTEKKVYSLPGQKFD 57
++S+AA+L +GK+ ++ K ++ SAT S + K EKKVYSL GQKFD
Sbjct: 77 TTSAAASLVKGKAQTTSTVVSLVKA------KTTSATVSLVKGKAKREKKVYSLAGQKFD 130
Query: 58 PPEEREPLRIFYESLSKQIPSSEMAEFWLMEHGLLSPERAKKAY 101
PPEEREPLRIFYESLSKQIP SEMAEFWLMEHG+LSPE+AK+A+
Sbjct: 131 PPEEREPLRIFYESLSKQIPGSEMAEFWLMEHGMLSPEKAKRAF 174
>AT1G19990.1 | chr1:6935327-6936665 FORWARD LENGTH=252
Length = 251
Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 36/52 (69%), Positives = 43/52 (82%)
Query: 49 YSLPGQKFDPPEEREPLRIFYESLSKQIPSSEMAEFWLMEHGLLSPERAKKA 100
Y LPGQK + P+ER+PLRIFYESL KQIP+S+MA+ WLME GLL E+AKK
Sbjct: 115 YDLPGQKREQPDERDPLRIFYESLYKQIPTSDMAQIWLMESGLLPAEKAKKV 166
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.308 0.123 0.338
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,166,744
Number of extensions: 81477
Number of successful extensions: 205
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 205
Number of HSP's successfully gapped: 2
Length of query: 101
Length of database: 11,106,569
Length adjustment: 71
Effective length of query: 30
Effective length of database: 9,160,033
Effective search space: 274800990
Effective search space used: 274800990
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 104 (44.7 bits)