BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0578000 Os05g0578000|AK065040
(271 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G62810.1 | chr5:25220323-25223571 FORWARD LENGTH=508 57 1e-08
>AT5G62810.1 | chr5:25220323-25223571 FORWARD LENGTH=508
Length = 507
Score = 56.6 bits (135), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 24/38 (63%), Positives = 30/38 (78%)
Query: 59 QSAVGFLKHPKVVASSDVQRRSFLEKKGLTVDEIDEAF 96
Q+A+ FL HP+V S + RRSFLE+KGLT +EIDEAF
Sbjct: 59 QNAIKFLSHPRVRGSPVIHRRSFLERKGLTKEEIDEAF 96
Score = 50.4 bits (119), Expect = 9e-07, Method: Compositional matrix adjust.
Identities = 25/60 (41%), Positives = 30/60 (50%)
Query: 145 PETESVDPVVPRHPKSYMEIMEMIQRGERXXXXXXXXXXXXXXXXXXSESRMAPKPKPWE 204
P D P HPKSYM+IM MIQRGE+ S+ R+APK KPW+
Sbjct: 313 PPAAPADSSAPPHPKSYMDIMSMIQRGEKPSNIREINDMPPNPNQPLSDPRIAPKSKPWD 372
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.305 0.122 0.333
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,792,923
Number of extensions: 152031
Number of successful extensions: 348
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 347
Number of HSP's successfully gapped: 2
Length of query: 271
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 174
Effective length of database: 8,447,217
Effective search space: 1469815758
Effective search space used: 1469815758
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)
S2: 111 (47.4 bits)