BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0547100 Os05g0547100|AK121657
         (145 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G17240.1  | chr4:9666799-9668292 REVERSE LENGTH=344             91   2e-19

>AT4G17240.1 | chr4:9666799-9668292 REVERSE LENGTH=344
          Length = 343

 Score = 91.3 bits (225), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 65/151 (43%), Positives = 79/151 (52%), Gaps = 39/151 (25%)

Query: 26  GGK--LPGLSRKLFQKAPSEHKKNALTEVKSGGNTRALAMVLRGE---------WVDEIA 74
           GGK  L  L RKLF K     K  ALTEVKS  NTR L+MVLR E            EIA
Sbjct: 100 GGKSTLGTLQRKLFGK----EKVKALTEVKS--NTRTLSMVLRSERELLGMNKDQEVEIA 153

Query: 75  ALRLQFEQKDIEVERLKDLCLWQREEIWTLKGAVFFPATQPDH----------------- 117
            L+ Q E+K+ EVE+LKDLCL QREEI +LK AV FP +                     
Sbjct: 154 ELKFQLEEKNREVEKLKDLCLKQREEIKSLKSAVLFPDSMNSQINQMQELNQAREIIPNL 213

Query: 118 -----NLTGQIQYLAEKIAVVKTKKHMPRSC 143
                +L GQ+Q +A+ +A VK  K++  SC
Sbjct: 214 QKQVISLNGQLQCIAQDLAEVKANKYLSESC 244



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.132    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,201,809
Number of extensions: 119024
Number of successful extensions: 284
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 281
Number of HSP's successfully gapped: 1
Length of query: 145
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 56
Effective length of database: 8,666,545
Effective search space: 485326520
Effective search space used: 485326520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 107 (45.8 bits)