BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0542200 Os05g0542200|AK071306
         (400 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G53050.2  | chr5:21510534-21513432 REVERSE LENGTH=397          440   e-124

>AT5G53050.2 | chr5:21510534-21513432 REVERSE LENGTH=397
          Length = 396

 Score =  440 bits (1131), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 222/399 (55%), Positives = 286/399 (71%), Gaps = 12/399 (3%)

Query: 1   MPYCEVDRYQNGDEWD----GVRLFYRRFGRGATKVLLVIGLAGTHDSWGPQIKGLTGSL 56
           MP+CEV +   G E       +++FYR +G G  K LL+IGLAGTH+SWGPQI GLTG+ 
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGT- 59

Query: 57  EPXXXXXXXXXXXXXXXXXXXXIEVCCFDNRGVGRSSVLPHKSYYSTVIMARDALALMDH 116
                                 IEVC FDNRG+GRSSV  HKS Y+T IMA D+++L+DH
Sbjct: 60  ---DKPNDDDDDDGGIVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMANDSISLLDH 116

Query: 117 LGWKKAHVFGHSMGAMISCKLAAMAPHRICSLALLNVTGGGFQCFPKLDGQMLSLAFRFL 176
           LGWKKAH+ GHSMGAMI+CKLAAMAP R+ SLALLNVTGGGF+CFPKLD + LS+A RFL
Sbjct: 117 LGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKSLSIAIRFL 176

Query: 177 RARTPEERALVDLETHYTKEYLDEKVGSCTRRTILYQEYVKGISSTGMQSNCGFEGQVNA 236
           +A+TPE+RA VDL+THY+K+YL+E VG+ TRR ILYQ+YVKGIS TGMQS  GF+GQ+NA
Sbjct: 177 KAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKYGFDGQINA 236

Query: 237 CWTHNMTTKELDTIRSAGFLVSVIHGRSDIIAQLCHARRLAERLIPVARMVELHGAHLVS 296
           CW H +T  E++ IRSAGFLVSVIHGR D+IAQ+C+ARRLA+RL PVARMV+LHG HLVS
Sbjct: 237 CWLHKITKVEIELIRSAGFLVSVIHGRHDVIAQICYARRLAQRLYPVARMVDLHGGHLVS 296

Query: 297 HERPEEVNNALMELIKATKSMMKPEEWSSQPENSSETGALISARPVSVTIRTDDGGNAAI 356
           HER EEVN AL+ELIKA++      +W++    + ET      R +++   + +G N A 
Sbjct: 297 HERTEEVNKALLELIKASEMKKISTDWTNL---TMETPGYFKRR-LTLIRSSTEGKNVAS 352

Query: 357 AVYNLLAKLQLSFLYVIGVILMGFEHMRNIVKVMKPVRV 395
             + +  K     L++ G++++ FE+ R   + + PV+V
Sbjct: 353 PAHFIAEKFHGFLLFLFGLLVLAFEYSRRAFRAVNPVKV 391



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,955,410
Number of extensions: 301153
Number of successful extensions: 713
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 711
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 299
Effective length of database: 8,337,553
Effective search space: 2492928347
Effective search space used: 2492928347
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 113 (48.1 bits)